This work describes a label-free electrochemical immunosensor for the sensing of Epstein-Barr virus (EBV) with high sensitivity. First, a monolayer of 1,6-hexanedithiol (HDT) was fabricated on the screen-printed electrode surface by the interaction between sulfur atoms and SPE. AuNPs can be modified on the electrode by the Au-S bond formed between the HDT-free group and Au atom in AuNPs. Protein A is then adsorbed onto AuNPs. Several parameters were optimized. The optimum concentration of protein A is 0.6 mg/mL. The optimum immobilization time for protein A is 90 min. The optimum concentration of antibody is 80 μg/mL. The optimum immobilization time for antibody is 90 min. Directional immobilization of EBV antibody is achieved by high affinity binding of protein A to the Fc segment of antibody. When antigen specifically binds to antibody, the formation of immune complexes blocks electron transfer of [Fe(CN)6]^4-/3- and is reflected in the detection of cyclic voltammetry/electrochemical impedance spectroscopy. The detection range is 1 pg/mL–l00 ng/mL with a LOD of 0.1 pg/mL. In addition, the proposed sensor exhibited an excellent antiinterference property.

中文翻译：

---

用于 Epstein-Barr 病毒检测的基于护理点的电化学免疫测定

这项工作描述了一种无标记的电化学免疫传感器，用于高灵敏度地感知 Epstein-Barr 病毒 (EBV)。首先，通过硫原子与 SPE 之间的相互作用，在丝网印刷电极表面制备单层 1,6-己二硫醇 (HDT)。AuNPs 可以通过在 AuNPs 中的无 HDT 基团和 Au 原子之间形成的 Au-S 键在电极上进行修饰。然后蛋白 A 被吸附到 AuNPs 上。优化了几个参数。蛋白 A 的最佳浓度为 0.6 mg/mL。蛋白质 A 的最佳固定时间为 90 分钟。抗体的最佳浓度为 80  μ克/毫升。抗体的最佳固定时间为 90 分钟。EBV 抗体的定向固定是通过蛋白 A 与抗体 Fc 片段的高亲和力结合来实现的。当抗原与抗体特异性结合时，免疫复合物的形成阻断了[Fe(CN)6] ^4-/3-的电子转移，并反映在循环伏安法/电化学阻抗谱的检测中。检测范围为 1 pg/mL–100 ng/mL，LOD 为 0.1 pg/mL。此外，所提出的传感器表现出优异的抗干扰性能。