Journal of Natural Medicines ( IF 2.5 ) Pub Date : 2022-05-31 , DOI: 10.1007/s11418-022-01627-0 Xiao Ye 1 , Xiao-Qian Liu 1 , Dong Zhang 1, 2 , Wei-Hong Feng 1 , Yao-Hua Liang 1 , Chun Li 1 , Zhi-Min Wang 1
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Abstract
The fruits of Gardenia jasminoides Ellis are an important herb medicine in Traditional Chinese Medicine (TCM) and have been used for thousands of years for clearing away heat and toxic materials. It mainly contains iridoids, pigments, organic acids, and flavonoids. Although belonging to one species, it has two kinds of cultivars and one variety widely distributed and sold. This study aims to develop an integrated and efficient analytical strategy for comprehensive profiling of phytochemicals and clarify the differences in all three populations. Based on reversed-phase ultra-high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry (UHPLC/ESI-QTOFMS), an optimized analytical approach for comprehensive profiling of phytochemicals in the fruits of G. jasminoides was established in negative ionization mode. The holistic metabolites profiling was carried out on UHPLC/ESI-QTOFMS and data analysis program Progenesis QI, and a total of 80 metabolites were obtained and interpreted by chromatographic and tandem mass spectral data. The interpretation of metabolites comprises iridoids, pigments, organic acids, and flavonoids. Principal component analysis and partial least square-discriminant analysis were performed, and 19 main different components could be obtained to distinguish the three populations. Combined with non-targeted and targeted data analysis, the integrated strategy developed in this study was feasibly applied to discern differences in the profiles of the phytochemicals accumulating in the fruits of three populations of G. jasminoides.
Graphical abstract
中文翻译：
使用液相色谱结合电喷雾电离四极杆飞行时间质谱法对栀子及其品种中的植物化学物质进行综合分析
摘要
栀子的果实是传统中药 (TCM) 中的重要草药，数千年来一直用于清热解毒。主要含有环烯醚萜类、色素、有机酸、类黄酮。虽属一个品种，但有两种栽培品种和一种品种，广为分布和销售。本研究旨在开发一种综合有效的分析策略，以全面分析植物化学物质，并阐明所有三个种群的差异。基于反相超高效液相色谱与电喷雾电离四极杆飞行时间质谱 (UHPLC/ESI-QTOFMS) 的结合，一种优化的分析方法，用于综合分析梨果实中的植物化学成分G. jasminoides采用负电离模式建立。在 UHPLC/ESI-QTOFMS 和数据分析程序 Progenesis QI 上进行整体代谢物分析，共获得 80 种代谢物并通过色谱和串联质谱数据进行解释。代谢物的解释包括环烯醚萜、色素、有机酸和类黄酮。进行了主成分分析和偏最小二乘判别分析，可以得到19个主要的不同成分来区分这三个群体。结合非靶向和靶向数据分析，本研究中开发的综合策略可可行地应用于辨别茉莉花三个种群果实中积累的植物化学成分的差异.
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