We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease resource consumption. The FS protocol can also use unique molecular identifiers (UMIs) for molecule counting while displaying reduced strand-invasion artifacts. FS will be especially useful for characterizing gene expression at high resolution across multiple samples.

中文翻译：

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使用 FLASH-seq 进行快速、高灵敏度的全长单细胞 RNA 测序

我们推出了 FLASH-seq (FS)，这是一种全长单细胞 RNA 测序 (scRNA-seq) 方法，与 Smart-seq3 相比，其灵敏度更高，操作时间更短。整个 FS 协议可以在约 4.5 小时内完成，易于自动化，并且可以轻松小型化以减少资源消耗。 FS 协议还可以使用独特的分子标识符 (UMI) 进行分子计数，同时显示减少的链侵入伪影。 FS 对于以高分辨率表征多个样本的基因表达尤其有用。