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Metabolomics-based mass spectrometry methods to analyze the chemical content of 3D organoid models
Analyst ( IF 3.6 ) Pub Date : 2022-05-30 , DOI: 10.1039/d2an00599a Shannon E Murphy 1 , Jonathan V Sweedler 1
Analyst ( IF 3.6 ) Pub Date : 2022-05-30 , DOI: 10.1039/d2an00599a Shannon E Murphy 1 , Jonathan V Sweedler 1
Affiliation
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Metabolomics, the study of metabolites present in biological samples, can provide a global view of sample state as well as insights into biological changes caused by disease or environmental interactions. Mass spectrometry (MS) is commonly used for metabolomics analysis given its high-throughput capabilities, high sensitivity, and capacity to identify multiple compounds in complex samples simultaneously. MS can be coupled to separation methods that can handle small volumes, making it well suited for analyzing the metabolome of organoids, miniaturized three-dimensional aggregates of stem cells that model in vivo organs. Organoids are being used in research efforts to study human disease and development, and in the design of personalized drug treatments. For organoid models to be useful, they need to recapitulate morphological and chemical aspects, such as the metabolome, of the parent tissue. This review highlights the separation- and imaging-based MS-based metabolomics methods that have been used to analyze the chemical contents of organoids. Future perspectives on how MS techniques can be optimized to determine the accuracy of organoid models and expand the field of organoid research are also discussed.
中文翻译：
基于代谢组学的质谱方法分析 3D 类器官模型的化学含量
代谢组学是对生物样品中存在的代谢物的研究，可以提供样品状态的全局视图以及对疾病或环境相互作用引起的生物变化的见解。质谱 (MS) 因其高通量、高灵敏度以及同时识别复杂样品中多种化合物的能力而常用于代谢组学分析。MS 可以与可处理小体积的分离方法结合使用，使其非常适合分析类器官的代谢组、体内建模的干细胞的微型三维聚集体器官。类器官被用于研究人类疾病和发育以及个性化药物治疗的设计。为了使类器官模型有用，它们需要概括母体组织的形态和化学方面，例如代谢组。本综述重点介绍了基于分离和成像的基于 MS 的代谢组学方法，这些方法已用于分析类器官的化学含量。还讨论了如何优化 MS 技术以确定类器官模型的准确性并扩展类器官研究领域的未来前景。
更新日期：2022-05-30
中文翻译：
基于代谢组学的质谱方法分析 3D 类器官模型的化学含量
代谢组学是对生物样品中存在的代谢物的研究，可以提供样品状态的全局视图以及对疾病或环境相互作用引起的生物变化的见解。质谱 (MS) 因其高通量、高灵敏度以及同时识别复杂样品中多种化合物的能力而常用于代谢组学分析。MS 可以与可处理小体积的分离方法结合使用，使其非常适合分析类器官的代谢组、体内建模的干细胞的微型三维聚集体器官。类器官被用于研究人类疾病和发育以及个性化药物治疗的设计。为了使类器官模型有用，它们需要概括母体组织的形态和化学方面，例如代谢组。本综述重点介绍了基于分离和成像的基于 MS 的代谢组学方法，这些方法已用于分析类器官的化学含量。还讨论了如何优化 MS 技术以确定类器官模型的准确性并扩展类器官研究领域的未来前景。
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