Since cancer has emerged as one of the most serious threats to human health, the highly sensitive determination of cancer cells is of significant importance to improve the accuracy of early clinical diagnosis. In our investigation, a novel cascade Fermat spiral microfluidic mixer chip (CFSMMC) combined with fluorescence sensors as a point-of-care (POC) testing system is successfully fabricated to detect and differentiate cancer cells (MCF-7) from normal cells with excellent sensitivity and selectivity. Here, copper ions (Cu²⁺) with peroxidase properties can catalyze the oxidation of the non-fluorescent substrate Amplex Red (AR) to the highly fluorescent resorufin (ox-AR) in the presence of hydrogen peroxide (H₂O₂). Subsequently, thanks to the quenching response of AS1411-AuNPs to ox-AR in the microchannel and the binding of AS1411 to nucleolin on the surface of cancer cells, a CFSMMC-based POC system is established for the highly sensitive detection and identification of human breast cancer cells in a “turn on” manner. The change in fluorescence intensity is linearly related to the concentration of MCF-7, ranging from 10² to 10⁷ cells per mL with a limit of detection (LOD) as low as 17 cells per mL. Interestingly, the cascaded AND logic gate is integrated with CFSMMC for the first time to distinguish cancer cells from normal cells under the control of logic functions, which exhibits great potential in the development of one-step rapid and intelligent detection and logic discrimination.

中文翻译：

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一种用于精确检测和逻辑鉴别癌细胞的级联费马螺旋微流控混合器芯片

由于癌症已成为对人类健康最严重的威胁之一，因此高灵敏度的癌细胞测定对于提高早期临床诊断的准确性具有重要意义。在我们的研究中，一种新型级联费马螺旋微流体混合器芯片 (CFSMMC) 结合荧光传感器作为护理点 (POC) 测试系统成功制造，用于检测和区分癌细胞 (MCF-7) 和正常细胞，具有优异的性能。灵敏度和选择性。在这里，具有过氧化物酶特性的铜离子 (Cu ^{2+ ) 可以在过氧化氢 (H} ₂ O ₂ ) 存在下催化非荧光底物 Amplex Red (AR) 氧化成高荧光试卤灵 (ox-AR)）。随后，由于AS1411-AuNPs对微通道中ox-AR的猝灭反应以及AS1411与癌细胞表面核仁素的结合，建立了基于CFSMMC的POC系统，用于高灵敏度检测和鉴定人乳腺。癌细胞以“开启”的方式。荧光强度的变化与 MCF-7 的浓度呈线性相关，范围从 10 ²到 10 ^{7 个}细胞/mL，检测限 (LOD) 低至 17 个细胞/mL。有趣的是，级联与逻辑门首次与CFSMMC集成，在逻辑函数的控制下区分癌细胞和正常细胞，在一步快速智能检测和逻辑判别方面展现出巨大潜力。