As a common gaseous signaling molecule, hydrogen sulfide (H₂S) plays a vital role in physiology and pathology. The development of fluorescent probes for detecting H₂S has attracted widespread attention. However, most of the reported fluorescent probes with nitrobenzoxadiazole (NBD) as the recognition group have been widely used to simultaneously detect biothiols and H₂S, instead of specifically detecting H₂S. Herein, a novel NBD-based near-infrared (NIR) fluorescent probe named CX-N for the detection of H₂S is synthesized. The selectivity of CX-N for H₂S is significantly higher than that for biothiols and other potential interferences. After reacting with H₂S, CX-N shows a significant increase in NIR fluorescence (75-fold), large Stokes shift (155 nm) and fast response (4 min). And the possible response mechanism of CX-N to H₂S is given and confirmed by HPLC and HRMS. Based on the low cytotoxicity of CX-N, it has been used for H₂S imaging in live cells and zebrafish. More importantly, CX-N has also been successfully applied for the real-time imaging of H₂S in inflammatory and tumor mice based on its NIR emission, which provides a reliable platform for the specific recognition of H₂S in complex biological systems.

中文翻译：

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一种基于硝基苯并恶二唑的近红外荧光探针，用于炎症和肿瘤小鼠中 H2S 的特异性成像

作为一种常见的气体信号分子，硫化氢（H ₂ S）在生理和病理中起着至关重要的作用。_{用于检测H 2} S的荧光探针的开发引起了广泛关注。然而，大多数报道的以硝基苯并恶二唑（NBD）为识别基团的荧光探针已被广泛用于同时检测生物硫醇和H ₂ S，而不是特异性检测H ₂ S。 ) 合成了用于检测H ₂ S 的荧光探针CX-N 。CX-N 对 H ₂ S 的选择性明显高于对生物硫醇和其他潜在干扰物的选择性。与H _2反应后S，CX-N 显示 NIR 荧光显着增加（75 倍）、大斯托克斯位移（155 nm）和快速响应（4 分钟）。并给出了CX-N对H ₂ S可能的反应机理，并通过HPLC和HRMS进行了证实。基于 CX-N 的低细胞毒性，它已被用于活细胞和斑马鱼的 H ₂ S 成像。更重要的是，CX-N还成功应用于基于近红外发射的炎症和肿瘤小鼠H ₂ S的实时成像，为复杂生物系统中H _{2 S的特异性识别提供了可靠的平台。}