Sucrose, one of the most widespread disaccharides in nature, has been available in daily human life for many centuries. As an abundant and cheap sweetener, sucrose plays an essential role in our diet and the food industry. However, it has been determined that many diseases, such as obesity, diabetes, hyperlipidemia, etc., directly relate to the overconsumption of sucrose. It arouses many explorations for the conversion of sucrose to high-value chemicals. Production of valuable substances from sucrose by chemical methods has been studied since a half-century ago. Compared to chemical processes, biotechnological conversion approaches of sucrose are more environmentally friendly. Many enzymes can use sucrose as the substrate to generate functional sugars, especially those from GH68, GH70, GH13, and GH32 families. In this review, enzymatic catalysis and whole-cell fermentation of sucrose for the production of valuable chemicals were reviewed. The multienzyme cascade catalysis and metabolic engineering strategies were addressed.

蔗糖是自然界中分布最广的二糖之一，数百年来一直存在于人类日常生活中。作为一种丰富而廉价的甜味剂，蔗糖在我们的饮食和食品工业中起着至关重要的作用。然而，已经确定许多疾病，如肥胖、糖尿病、高脂血症等，都与蔗糖的过量摄入直接相关。它引发了蔗糖转化为高价值化学品的许多探索。半个世纪前，人们一直在研究通过化学方法从蔗糖生产有价值的物质。与化学过程相比，蔗糖的生物技术转化方法更环保。许多酶可以使用蔗糖作为底物来生成功能性糖，尤其是来自 GH68、GH70、GH13 和 GH32 家族的糖。在本次审查中，综述了用于生产有价值化学品的蔗糖的酶催化和全细胞发酵。解决了多酶级联催化和代谢工程策略。