Cadmium accumulation regulated by a rice heavy-metal importer is harmful for host plant and leaf bacteria,Journal of Advanced Research

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Cadmium accumulation regulated by a rice heavy-metal importer is harmful for host plant and leaf bacteria
Journal of Advanced Research ( IF 11.4 ) Pub Date : 2022-05-29 , DOI: 10.1016/j.jare.2022.05.010
Jingjing Tian ₁ , Li Wang ₁ , Shugang Hui ₁ , Dan Yang ₁ , Yuqing He ₂ , Meng Yuan ₂

Affiliation

Introduction

Cadmium (Cd), one of the major toxic heavy metals, causes severe deleterious effects on all living organisms from prokaryotes to eukaryotes. Cadmium deposition affects bacterial diversity and bacterial population in soil. Cadmium accumulation in plants is mainly controlled by transporters and the resulting Cd enrichment gives rise to phytotoxicity.

Objective

This study aimed to mine transporters that control Cd import or accumulation in rice and uncover the underlying mechanisms that how accumulated Cd poses risks to host plant and leaf bacteria.

Methods

RNA-seq analysis, histochemical assays, and elemental quantification were carried out to reveal the biological roles of OsABCG43 for Cd import. Pathogen inoculation, IC₅₀ value, and bacterial virulence assays were conducted to disclose the effects of Cd on leaf bacteria.

Results

OsABCG43 is characterized as a Cd importer controlling Cd accumulation in rice. OsABCG43 was induced under Cd stress and specifically expressed in the vasculature of leaves and roots. Overexpression of OsABCG43 caused Cd accumulation which inhibits photosynthesis and development and alters the antioxidant system, resulting in phytotoxicity. Moreover, overexpression of OsABCG43 resulted in retarded plant growth and enhanced rice sensitivity to Cd stress. Numerous differentially expressed genes were identified via RNA-seq analysis between the OsABCG43-overexpressing plants and wild type, which functioned in Cd or reactive oxygen species (ROS) homeostasis. In addition, OsABCG43 transcripts were induced by leaf bacteria Xanthomonas oryzae pv. oryzicola (Xoc) and X. oryzae pv. oryzae (Xoo). The enriched Cd directly impaired the formation of virulence factors for the leaf bacteria, preventing colonization or proliferation of Xoc or Xoo in rice leaves.

Conclusion

This work reveals that OsABCG43 is expressed specifically in the vascular and plasma membrane-localized OsABCG43 functions as a Cd importer. OsABCG43-mediated import of Cd is harmful for both rice and the corresponding leaf bacteria.

中文翻译：

水稻重金属进口商调节的镉积累对寄主植物和叶片细菌有害

介绍

镉 (Cd) 是主要有毒重金属之一，会对从原核生物到真核生物的所有生物体造成严重的有害影响。镉沉积影响土壤中的细菌多样性和细菌种群。植物中镉的积累主要受转运蛋白控制，由此产生的镉富集会引起植物毒性。

客观的

本研究旨在挖掘控制水稻中 Cd 导入或积累的转运蛋白，并揭示积累的 Cd 如何对寄主植物和叶片细菌构成风险的潜在机制。

方法

进行 RNA-seq 分析、组织化学分析和元素定量以揭示 OsABCG43 对 Cd 输入的生物学作用。进行病原体接种、IC ₅₀值和细菌毒力测定以揭示 Cd 对叶细菌的影响。

结果

OsABCG43 被表征为控制水稻中 Cd 积累的 Cd 输入蛋白。OsABCG43在 Cd 胁迫下被诱导，并在叶和根的脉管系统中特异性表达。OsABCG43的过表达导致 Cd 积累，抑制光合作用和发育并改变抗氧化系统，导致植物毒性。此外，OsABCG43的过表达导致植物生长迟缓并增强了水稻对镉胁迫的敏感性。通过OsABCG43过表达植物和野生型之间的 RNA-seq 分析鉴定了许多差异表达的基因，这些基因在 Cd 或活性氧 (ROS) 稳态中发挥作用。此外，OsABCG43转录物由叶细菌米黄单胞菌 pv诱导。oryzicola ( Xoc ) 和X. oryzae pv。米( Xoo )。富集的 Cd 直接破坏了叶片细菌毒力因子的形成，阻止了Xoc或Xoo在水稻叶片中的定殖或增殖。