Cell migration on an adhesive substrate surface comprises actin-based protrusion at the front and retraction of the tail in combination with coordinated adhesion to, and detachment from, the substrate. To study the effect of cell-to-substrate adhesion on the chemotactic response of Dictyostelium discoideum cells, we exposed the cells to patterned substrate surfaces consisting of adhesive and inert areas, and forced them by a gradient of chemoattractant to enter the border between the two areas. Wild-type as well as myosin II-deficient cells stop at the border of an adhesive area. They do not detach with their rear part, while on the nonadhesive area they protrude pseudopods at their front toward the source of chemoattractant. Avoidance of the nonadhesive area may cause a cell to move in tangential direction relative to the attractant gradient, keeping its tail at the border of the adhesive surface.

粘附基质表面上的细胞迁移包括前端基于肌动蛋白的突出和尾部的缩回，以及与基质的协调粘附和脱离。研究细胞与基质粘附对盘基网柄菌趋化反应的影响细胞，我们将细胞暴露于由粘合剂和惰性区域组成的图案化基底表面，并通过化学引诱剂的梯度迫使它们进入两个区域之间的边界。野生型和肌球蛋白 II 缺陷型细胞停在粘附区域的边界。它们的后部不会脱离，而在非粘附区域，它们的前部向化学引诱剂的来源伸出伪足。避开非粘附区域可能会导致细胞相对于引诱剂梯度沿切线方向移动，将其尾部保持在粘附表面的边界处。