Okra is a kind of flavonoid-rich food which was reported to have a variety of health functions. Flavonoids are the major polyphenolic compounds in okra and are thought to play a role in reducing the risk of disease. The aim of this study was to isolate and identify the flavonoids composition in okra pods and explore the activity of the main flavonoids components identified on inhibiting tumor cell proliferation in vitro. Six individual flavonoids were identified by HPLC-MS/MS: quercetin-3-gentiobioside (Q3G), quercetin-3-sambubioside (Q3S), rutin, quercetin-7-glucoside (Q7G), isoquercitrin (ISO) and quercetin-3-malonylglucoside (Q3M), which were all separated well within 30 min. The analytical method was validated by the recovery of spiked samples and so on. Moreover, four main flavonoids components, namely Q3G, Q3S, ISO and Q3M, exhibited significant (p < 0.05) inhibition of NCI-N87, A375, A549 cells proliferation (25−100 μmol/L) and of HFLS-RA (200−300 μmol/L) in different levels, according to MTT method, respectively. It is demonstrated that the flavonoids components of okra exhibited a noteworthy development prospect as a possible nutraceutical dietary supplement.

中文翻译：

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黄秋葵 (Abelmoschus esculentus L. Moench) 中黄酮类化合物的鉴定和定量以及鉴定的四种主要成分的体外抗增殖活性

秋葵是一种富含黄酮类化合物的食品，据报道具有多种保健功能。黄酮类化合物是秋葵中的主要多酚化合物，被认为在降低疾病风险方面发挥作用。本研究的目的是分离和鉴定秋葵荚中的黄酮类化合物成分，并探索鉴定的主要黄酮类化合物在体外抑制肿瘤细胞增殖的活性。通过 HPLC-MS/MS 鉴定出六种单独的黄酮类化合物：槲皮素 3-龙胆双糖苷 (Q3G)、槲皮素 3-三丁二糖苷 (Q3S)、芦丁、槲皮素 7-葡萄糖苷 (Q7G)、异槲皮素 (ISO) 和槲皮素 3-丙二酰葡糖苷 (Q3M)，它们在 30 分钟内得到了很好的分离。通过加标回收率等对分析方法进行了验证。此外，四种主要的类黄酮成分，即 Q3G、Q3S、ISO 和 Q3M，根据 MTT 法，p < 0.05) 分别以不同水平抑制 NCI-N87、A375、A549 细胞增殖 (25-100 μmol/L) 和 HFLS-RA (200-300 μmol/L) 。证明秋葵中的类黄酮成分作为一种可能的营养保健品具有显着的发展前景。