Purpose

Atractylodes lancea is a medicinal plant widely used in treating rheumatic diseases, digestive disorders, night blindness, and influenza. Microbes greatly impact plant growth and metabolism. However, the microbiome associated with A. lancea remains unclear. Hence, we aimed at assessing the effect of soil microbe inoculation on A. lancea under heat stress from multiple perspectives, including regulation of growth, valuable secondary metabolites, root endophytic and rhizosphere bacterial communities.

Methods

A. lancea was inoculated with soil microbes, then grown under normal/high temperature. Biomass, chlorophyll contents, production of major medicinal compounds, physiochemical properties of the soil, and in the composition of root bacterial communities of A. lancea were investigated.

Results

Soil microbe inoculation promoted root sink strength, accumulation of medicinal compounds, and attenuated damage caused by heat stress. A. lancea showed preference for the endophytic bacterial genera Rhodococcus, Ralstonia, Dongia Paenibacillus and Burkholderia-Caballeronia-Paraburkholderia post-inoculation, the latter four genera playing important roles in protection from heat stress, with abundance of the latter two specifically positively correlated to medicinal compound production. A. lancea enriched the bacterial genera Saccharimonadales, Novosphingobium and excluded Chitinophaga in its rhizosphere post-inoculation.

Conclusions

Soil microbes characteristically promoted A. lancea growth, improved heat stress tolerance, and promoted root medicinal compound accumulation. A. lancea selectively enriched particular endophytic and rhizospheric bacterial communities post-inoculation, possibly due to unique aromatic root exudates. The selected bacteria potentially synergistically improved soil available nutrients and uptake by root. Bacterial species selected by A. lancea root have the potential to serve as biological fertilizers for A. lancea farming.

中文翻译：

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土壤微生物接种改变细菌群落促进热胁迫下白术根系生长

目的

白术是一种药用植物，广泛用于治疗风湿病、消化系统疾病、夜盲症和流感。微生物极大地影响植物的生长和新陈代谢。然而，与A. lancea相关的微生物组仍不清楚。因此，我们旨在从多个角度评估土壤微生物接种对高温胁迫下杉木的影响，包括生长调节、有价值的次生代谢产物、根内生菌和根际细菌群落。

方法

A. lancea接种土壤微生物，然后在常温/高温下生长。研究了生物量、叶绿素含量、主要药用化合物的产生、土壤的理化性质以及矛头草根系细菌群落的组成。

结果

土壤微生物接种促进了根库强度，药物化合物的积累，减轻了热应激造成的损害。A. lancea对接种后的内生细菌属Rhodococcus、Ralstonia、Dongia Paenibacillus和Burkholderia-Caballeronia-Paraburkholderia表现出偏好，后四个属在防止热应激方面发挥重要作用，后两者的丰度与药用呈正相关复合生产。A. lancea富集了细菌属Saccharimonadales , Novosphingobium 并排除了几丁质噬菌体在接种后的根际。

结论

土壤微生物具有促进杉木生长、提高耐热胁迫耐受性和促进根部药用化合物积累的特征。A. lancea在接种后选择性地富集了特定的内生和根际细菌群落，这可能是由于独特的芳香根分泌物。选定的细菌可能协同改善土壤有效养分和根系的吸收。A. lancea根部选择的细菌物种有潜力作为A. lancea农业的生物肥料。