Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
A portable blood lactate sensor with a non-immobilized enzyme for early sepsis diagnosis
Analyst ( IF 3.6 ) Pub Date : 2022-05-26 , DOI: 10.1039/d2an00218c Patcharakorn Kiatamornrak 1, 2 , Siraprapa Boobphahom 3 , Tanat Lertussavavivat 1, 2, 4 , Pranee Rattanawaleedirojn 3 , Orawon Chailapakul 5 , Nadnudda Rodthongkum 3 , Nattachai Srisawat 1, 2, 4, 6
Analyst ( IF 3.6 ) Pub Date : 2022-05-26 , DOI: 10.1039/d2an00218c Patcharakorn Kiatamornrak 1, 2 , Siraprapa Boobphahom 3 , Tanat Lertussavavivat 1, 2, 4 , Pranee Rattanawaleedirojn 3 , Orawon Chailapakul 5 , Nadnudda Rodthongkum 3 , Nattachai Srisawat 1, 2, 4, 6
Affiliation
|
Early determination of blood lactate levels may accelerate the detection of sepsis, one of the most time-sensitive illnesses. We developed and validated a portable blood lactate detection kit for clinical screening that can measure early bedside lactate levels in intensive care unit (ICU) patients suspected of having sepsis. A TiO2 sol–G nanocomposite was prepared and coated on a screen-printed carbon electrode (SPCE) integrated with non-immobilized lactate oxidase (LOx) to produce a novel lactate biosensor with high sensitivity and high storage stability for human blood lactate measurement. The detection kit was based on an electrochemical technique and showed a wide linear range of 1–20 mM (R2 = 0.9937) with a low detection limit of 0.2 mM for lactate detection. This allowed for differentiating patient groups who may have sepsis using a cut-off level of 4 mM. The device was successfully implemented for blood lactate determination in critical patients, showing an accuracy range from 75% to 112%. This device provided high-precision and rapid quantitative information validated using a blood gas analyzer. Our detection kit might help to reduce the morbidity and mortality rates in severe sepsis and septic shock patients in community hospitals.
中文翻译：
用于早期脓毒症诊断的带有非固定化酶的便携式血乳酸传感器
早期测定血乳酸水平可能会加速脓毒症的检测，脓毒症是对时间最敏感的疾病之一。我们开发并验证了一种用于临床筛查的便携式血乳酸检测试剂盒，该试剂盒可以测量疑似脓毒症重症监护病房 (ICU) 患者的早期床边乳酸水平。制备了一种 TiO 2 sol-G 纳米复合材料，并将其涂覆在与非固定化乳酸氧化酶 (LOx) 结合的丝网印刷碳电极 (SPCE) 上，从而制备出一种用于人血乳酸测量的高灵敏度和高储存稳定性的新型乳酸生物传感器。该检测试剂盒基于电化学技术，具有 1-20 mM ( R 2= 0.9937)，乳酸检测的低检测限为 0.2 mM。这允许使用 4 mM 的截止水平来区分可能患有败血症的患者组。该装置成功应用于危重患者的血乳酸测定，准确率在 75% 到 112% 之间。该设备提供了使用血气分析仪验证的高精度和快速的定量信息。我们的检测试剂盒可能有助于降低社区医院严重败血症和感染性休克患者的发病率和死亡率。
更新日期：2022-05-26
中文翻译：
用于早期脓毒症诊断的带有非固定化酶的便携式血乳酸传感器
早期测定血乳酸水平可能会加速脓毒症的检测，脓毒症是对时间最敏感的疾病之一。我们开发并验证了一种用于临床筛查的便携式血乳酸检测试剂盒，该试剂盒可以测量疑似脓毒症重症监护病房 (ICU) 患者的早期床边乳酸水平。制备了一种 TiO 2 sol-G 纳米复合材料，并将其涂覆在与非固定化乳酸氧化酶 (LOx) 结合的丝网印刷碳电极 (SPCE) 上，从而制备出一种用于人血乳酸测量的高灵敏度和高储存稳定性的新型乳酸生物传感器。该检测试剂盒基于电化学技术，具有 1-20 mM ( R 2= 0.9937)，乳酸检测的低检测限为 0.2 mM。这允许使用 4 mM 的截止水平来区分可能患有败血症的患者组。该装置成功应用于危重患者的血乳酸测定，准确率在 75% 到 112% 之间。该设备提供了使用血气分析仪验证的高精度和快速的定量信息。我们的检测试剂盒可能有助于降低社区医院严重败血症和感染性休克患者的发病率和死亡率。
京公网安备 11010802027423号