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Effect of green synthesized ZnO nanoparticles using Paspalum scrobiculatum grains extract in biological applications
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2022-05-25 , DOI: 10.1002/jemt.24167
K Velsankar 1 , G Parvathy 1 , S Mohandoss 2 , Sakkarapani Sudhahar 1
Affiliation  

In this report, ZnO nanoparticles were biosynthesized using Paspalum scrobiculatum grains extract for the first time. GC–MS analysis explicated that diethyl phthalate was the major phytocompound with 94.09% in aqueous extract. ZnO nanoparticles formation was confirmed by various physicochemical analyses. HR-TEM images showed the hexagonal, rectangular shaped nanoparticles in 15–30 nm size. The antioxidant, anti-inflammatory, and anti-diabetic analyses showed the effective bioactivity of ZnO nanoparticles in 80 μg/ml concentration with 95.36%, 94.08%, and 91.96%, respectively. The morphological and tissue changes witnessed in larvicidal and insecticidal activities against Culex tritaeniorhynchus and Tribolium castaneum revealed the efficient nature of ZnO nanoparticles in 100 ppm at 48 h and 100 μg/kg at 72 h, respectively. The morphological changes in antibacterial activity demonstrated the bactericidal nature of ZnO nanoparticles against Salmonella typhi and Staphylococcus aureus in 150 μg/ml concentration. The morphological observations in anticancer activity against HepG2 liver cancer cells showed the potent drug features of ZnO nanoparticles in 100 μg/ml concentration with 97.18% of cytotoxicity. The ZnO nanoparticles showed no toxicity against HDF normal cells in lower concentrations and it explicated the biocompatible features of nanoparticles. The Vigna radiata plant growth was efficiently promoted by low (60 ppm) concentration of nanoparticles. The ZnO nanoparticles divulged effective degradation of IPA, EDTA, BQ, and DPBF in 75%, 45%, 55%, and 80% through ROS formation, respectively. Thus, the synthesized ZnO nanoparticles are biocompatible and inexpensive material compared to the traditional one and can be utilized as an efficient material in biological fields.

中文翻译:

雀稗籽粒提取物绿色合成氧化锌纳米粒子在生物应用中的作用

在本报告中,首次使用雀稗籽粒提取物生物合成 ZnO 纳米颗粒。GC-MS 分析表明邻苯二甲酸二乙酯是主要的植物化合物,在水提取物中占 94.09%。通过各种物理化学分析证实了 ZnO 纳米颗粒的形成。HR-TEM 图像显示尺寸为 15-30 nm 的六边形矩形纳米颗粒。抗氧化、抗炎和抗糖尿病分析表明,80 μg/ml 浓度的 ZnO 纳米颗粒的有效生物活性分别为 95.36%、94.08% 和 91.96%。杀幼虫和杀虫活动对三带喙库蚊赤谷栗的形态和组织变化揭示了 ZnO 纳米颗粒在 48 小时时 100 ppm 和 72 小时时分别为 100 μg/kg 的有效性质。抗菌活性的形态变化证明了150 μg/ml 浓度的 ZnO 纳米颗粒对伤寒沙门氏菌金黄色葡萄球菌具有杀菌作用。对HepG2肝癌细胞的抗癌活性形态学观察表明,100 μg/ml浓度的ZnO纳米颗粒具有97.18%的细胞毒性,具有很强的药物特性。ZnO 纳米粒子在较低浓度下对 HDF 正常细胞没有毒性,这说明了纳米粒子的生物相容性特征。豇豆辐射低浓度(60 ppm)的纳米粒子有效地促进了植物的生长。ZnO 纳米粒子通过 ROS 的形成分别显示了 75%、45%、55% 和 80% 的 IPA、EDTA、BQ 和 DPBF 的有效降解。因此,合成的 ZnO 纳米粒子与传统纳米粒子相比具有生物相容性且价格低廉,可用作生物领域的有效材料。
更新日期:2022-05-25
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