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d-Amino Acid-Based Antifouling Peptides for the Construction of Electrochemical Biosensors Capable of Assaying Proteins in Serum with Enhanced Stability
ACS Sensors ( IF 8.2 ) Pub Date : 2022-05-26 , DOI: 10.1021/acssensors.2c00518 Shuju Zhao 1 , Xiujuan Qiao 1 , Min Chen 1 , Yanxin Li 1 , Xin Wang 1 , Zhenying Xu 1 , Yumin Wu 1 , Xiliang Luo 1
ACS Sensors ( IF 8.2 ) Pub Date : 2022-05-26 , DOI: 10.1021/acssensors.2c00518 Shuju Zhao 1 , Xiujuan Qiao 1 , Min Chen 1 , Yanxin Li 1 , Xin Wang 1 , Zhenying Xu 1 , Yumin Wu 1 , Xiliang Luo 1
Affiliation
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The susceptibility of peptides to proteolytic degradation in human serum significantly hindered the potential application of peptide-based antifouling biosensors for long-term assaying of clinical samples. Herein, a robust antifouling biosensor with enhanced stability was constructed based on peptides composed of d-amino acids (d-peptide) with prominent proteolytic resistance. The electrode was electropolymerized with poly(3,4-ehtylenedioxythiophene) and electrodeposited with Au nanoparticles (AuNPs), and the d-peptide was then immobilized onto the AuNPs, and a typical antibody specific for immunoglobulin M (IgM) was immobilized. Because of the effect of d-amino acids, the d-peptide-modified electrode surface showed prominent antifouling capability and high tolerance to enzymatic hydrolysis. Moreover, the d-peptide-modified electrode exhibited much stronger long-term stability, as well as antifouling ability in human serum than the electrode modified with normal peptides. The electrochemical biosensor exhibited a sensitive response to IgM linearly within the range of 100 pg mL–1 to 1.0 μg mL–1 and a very low detection limit down to 37 pg mL–1, and it was able to detect IgM in human serum with good accuracy. This work provided a new strategy to develop robust peptide-based biosensors to resist the proteolytic degradation for practical application in complex clinical samples.
中文翻译：
d-氨基酸基防污肽用于构建电化学生物传感器，能够检测血清中的蛋白质并提高稳定性
肽对人血清中蛋白水解降解的敏感性显着阻碍了基于肽的防污生物传感器在临床样品长期分析中的潜在应用。在此，基于具有突出的蛋白水解抗性的d-氨基酸（d-肽）组成的肽构建了一种具有增强稳定性的坚固防污生物传感器。电极与聚（3,4-亚乙撑二氧噻吩）电聚合并用金纳米粒子（AuNPs）电沉积，然后将d-肽固定在AuNPs上，并固定典型的免疫球蛋白M（IgM）特异性抗体。由于d-氨基酸的作用，d-肽修饰的电极表面表现出显着的防污能力和对酶水解的高耐受性。此外，与普通肽修饰的电极相比， d-肽修饰的电极表现出更强的长期稳定性，以及在人血清中的防污能力。电化学生物传感器在 100 pg mL –1至 1.0 μg mL –1范围内对 IgM 表现出线性敏感响应，检测限极低，低至 37 pg mL –1，它能够检测人血清中的 IgM良好的准确性。这项工作提供了一种新的策略来开发强大的基于肽的生物传感器来抵抗蛋白水解降解，以便在复杂的临床样本中实际应用。
更新日期：2022-05-26
中文翻译：
d-氨基酸基防污肽用于构建电化学生物传感器，能够检测血清中的蛋白质并提高稳定性
肽对人血清中蛋白水解降解的敏感性显着阻碍了基于肽的防污生物传感器在临床样品长期分析中的潜在应用。在此，基于具有突出的蛋白水解抗性的d-氨基酸（d-肽）组成的肽构建了一种具有增强稳定性的坚固防污生物传感器。电极与聚（3,4-亚乙撑二氧噻吩）电聚合并用金纳米粒子（AuNPs）电沉积，然后将d-肽固定在AuNPs上，并固定典型的免疫球蛋白M（IgM）特异性抗体。由于d-氨基酸的作用，d-肽修饰的电极表面表现出显着的防污能力和对酶水解的高耐受性。此外，与普通肽修饰的电极相比， d-肽修饰的电极表现出更强的长期稳定性，以及在人血清中的防污能力。电化学生物传感器在 100 pg mL –1至 1.0 μg mL –1范围内对 IgM 表现出线性敏感响应，检测限极低，低至 37 pg mL –1，它能够检测人血清中的 IgM良好的准确性。这项工作提供了一种新的策略来开发强大的基于肽的生物传感器来抵抗蛋白水解降解，以便在复杂的临床样本中实际应用。
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