An in vitro pollen germination protocol for Triticale, the man-made cereal, has been reported. Triticale pollen may be classified as recalcitrant pollen as it is difficult to germinate on artificial medium. Using this pollen germination medium (PGM), viability and pollen vigour of triticale lines may be tested within 10–15 min. PGM used for triticale consisted of 19% maltose, 15% polyethylene glycol 4000, 60 mgl⁻¹ boric acid, 20 mgl⁻¹ calcium nitrate, 80 mgl⁻¹ potassium nitrate, 160 mgl⁻¹ magnesium sulphate and 1% agar. This PGM has been altered slightly which supported pollen germination of other genotypes. This complex medium contains many inorganic salts but in low concentration. The PGM showed > 93% pollen germination with intact pollen tubes at 17 ± 1 °C and pH 6.8. During the flowering period, a pollen sterility of less than 10% was recorded. Triticale pollen was viable more than 1.45 h which is much longer time as compared to wheat. This tool would be useful in triticale breeding especially in wide crosses with wheat or rye.

已经报道了人工谷物黑小麦的体外花粉萌发方案。黑小麦花粉可归类为顽固性花粉，因为它难以在人工培养基上发芽。使用这种花粉萌发培养基 (PGM)，可在 10-15 分钟内测试小黑麦系的活力和花粉活力。用于黑小麦的 PGM 由 19% 麦芽糖、15% 聚乙二醇 4000、60 mgl ^-1硼酸、20 mgl ^-1硝酸钙、80 mgl ^-1硝酸钾、160 mgl ^-1硫酸镁和 1% 琼脂。该 PGM 已略有改变，支持其他基因型的花粉萌发。这种复杂的介质含有许多无机盐，但浓度很低。PGM 在 17 ± 1 °C 和 pH 值 6.8 下显示 > 93% 的花粉发芽率和完整的花粉管。在开花期间，记录到花粉不育率低于 10%。黑小麦花粉的存活时间超过 1.45 小时，这比小麦长得多。该工具可用于小黑麦育种，尤其是与小麦或黑麦的广泛杂交。