Benzotriazole ultraviolet stabilizers (BZT-UVs) are added to various products to prevent damage caused by UV light and have emerged as contaminants of concern. Although BZT-UVs are detected in aquatic biota globally, few studies have assessed their potential toxic effects. The objective of the present study was to assess effects of 2-(2H-Benzotriazol-2-yl)-4-methylphenol (UV-P) on reproductive success of Japanese medaka (Oryzias latipes) in a standard 21-day reproduction assay. Japanese medaka were exposed to dietary UV-P at concentrations of 0, 36, 158, and 634 ng UV-P/g food, for a total of 28 days which included 7 days of exposure prior to the start of the 21-day reproduction assay. No significant effect on egg production or fertilization success was observed. Abundances of transcripts of erα, vtgI, cyp1a, or cyp3a4 were not significantly different in livers from male or female fish exposed to UV-P. However, abundances of transcripts of cyp11a and cyp19a were significantly lower in gonads from female fish. There was a trend of increasing concentrations of E2 and a non-significant increase of T in the 634 ng/g treatment in plasma from female fish exposed to UV-P. Concentrations of 11-KT were unchanged in plasma from males exposed to UV-P. These responses suggest weak perturbation of steroidogenesis, consistent with an antiandrogenic mode of action. However, this perturbation was insufficient to impair reproductive performance. Metabolomics analysis of female livers suggests altered concentrations of various metabolites and biological pathways, including glutathione metabolism, suggesting that UV-P might cause responses related to oxidative stress or phase II metabolism. However, metabolomics revealed no obvious mechanism of toxicity. Overall, results of this study indicate that dietary exposure to UV-P up to 634 ng/g food does not significantly impact reproductive performance of Japanese medaka but impacts on steroidogenesis could indicate a potential mechanism of toxicity which might lead to reproductive impairment in more sensitive species.

苯并三唑紫外线稳定剂 (BZT-UV) 被添加到各种产品中，以防止紫外线造成的损害，并已成为令人担忧的污染物。尽管在全球水生生物群中都检测到 BZT-UV，但很少有研究评估其潜在的毒性作用。本研究的目的是在标准的 21 天繁殖试验中评估 2-(2H-Benzotriazol-2-yl)-4-methylphenol (UV-P) 对日本青鳉 ( Oryzias latipes ) 繁殖成功率的影响。日本青鳉暴露于 0、36、158 和 634 ng UV-P/g 食物浓度的膳食 UV-P，共 28 天，其中包括 21 天繁殖开始前的 7 天暴露化验。没有观察到对产卵或受精成功的显着影响。丰富的成绩单erα、vtgI、cyp1a或cyp3a4在暴露于 UV-P 的雄性或雌性鱼的肝脏中没有显着差异。然而，cyp11a和cyp19a的转录本丰度雌性鱼的性腺显着降低。在暴露于 UV-P 的雌鱼血浆中，在 634 ng/g 处理中，E2 浓度有增加的趋势，而 T 没有显着增加。暴露于 UV-P 的男性血浆中 11-KT 的浓度没有变化。这些反应表明类固醇生成的微弱扰动，与抗雄激素作用模式一致。然而，这种扰动不足以损害生殖性能。女性肝脏的代谢组学分析表明，各种代谢物和生物途径的浓度发生了变化，包括谷胱甘肽代谢，这表明 UV-P 可能引起与氧化应激或 II 期代谢相关的反应。然而，代谢组学没有发现明显的毒性机制。全面的，