Marine oil spills have been widely reported, and bioremediation is proposed to be an environmentally friendly approach to clean these spills. However, maintaining a high biomass of oil-degrading bacteria has proved challenging. Here, a carrier that enables simultaneous bacterial immobilisation and oil absorption is proposed as a solution. Porous cellulose triacetate beads (PCTBs) derived from cellulose triacetate powder were obtained through the acetylation of kapok fibres. This study revealed, that 12.5 and 16.67% w/v PCTBs precipitated in distilled water showed crude oil absorbency of 2.4850 ± 0.1326 and 1.9852 ± 0.2343 g/g, respectively. Reusability tests of PCTBs showed promising results with no reduction in oil absorption after five reabsorption cycles. Successful immobilisation of the oil-degrading bacteria Acinetobacter venetianus on PCTBs was achieved, and enumeration of bacteria showed no statistically significant differences compared with those on kapok fibres despite the raw fibres having a larger surface area. The scanning electron microscopy analysis revealed that A. venetianus adhered to the outer and inner walls of the beads through secreted exopolysaccharides. PCTBs with oil-degrading bacteria are potentially able to absorb and degrade oil simultaneously, thus highlighting the novelty of a working alternative for sustainable oil spill mitigation.

中文翻译：

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木棉棉纤维改性成多孔三醋酸纤维素珠用于溢油修复

海洋石油泄漏已被广泛报道，生物修复被认为是一种清洁这些泄漏的环保方法。然而，事实证明，保持石油降解细菌的高生物量具有挑战性。在这里，提出了一种能够同时固定细菌和吸油的载体作为解决方案。通过木棉纤维的乙酰化获得源自三乙酸纤维素粉末的多孔三乙酸纤维素珠（PCTB）。该研究表明，在蒸馏水中沉淀的 12.5% 和 16.67% w/v PCTB 的原油吸光度分别为 2.4850 ± 0.1326 和 1.9852 ± 0.2343 g/g。PCTB 的可重复使用性测试显示出令人鼓舞的结果，在五个再吸收循环后吸油量没有减少。成功固定化油降解菌实现了 PCTBs 上的威尼斯不动杆菌，尽管原纤维具有更大的表面积，但与木棉纤维相比，细菌计数没有统计学上的显着差异。扫描电子显微镜分析表明，A. venetianus通过分泌的胞外多糖粘附在珠子的外壁和内壁上。具有石油降解细菌的 PCTB 可能能够同时吸收和降解石油，从而突出了可持续石油泄漏缓解工作替代方案的新颖性。