ABSTRACT

Dimethyl sulfoxide (DMSO) is widely used as a vehicle solvent in ecotoxicity bioassays.Despite its frequent use, it could be toxic for organisms at some concentrations. Hence, this study aims to investigate the effects of DMSO on the model organisms: microalgae Raphidocelis subcapitata, microcrustacean Daphnia magna, and rotifer Brachionus calyciflorus. DMSO was applied to the organisms in concentrations ranging from 0.031% to 4%. For R. subcapitata the lowest observed effect concentration (LOEC) after 72 h of exposure was 0.125% DMSO. The 50% effective concentration (EC₅₀) was 2.138 ± 0.372%. In D. magna, significant differences in the mobility after 24 h or 48 h of exposure were observed for 1% DMSO being 1.712 ± 0.207% and 1.167 ± 0.220% DMSO the EC₅₀ observed for 24 h and 48 h exposure, respectively. For B. calyciflorus, it was not possible to validate the tests performed, as there were insufficient animals alive in the control conditions at the end of the exposure period. Therefore, we recommended avoiding DMSO as a vehicle in assays using B. calyciflorus, and to use final DMSO concentrations in an experimental solution not exceeding 0.125% for R. subcapitata and 0.5% for D. magna. Finally, using a maximum of 0.01% DMSO is safe for the organisms tested.

The effects of the vehicle solvent dimethyl sulfoxide (DMSO) were investigated in three aquatic model organisms, the microalgae Raphidocelis subcapitata, the microcrustacean Daphnia magna and the rotifer Brachionus calyciflorus. As a result, negative effects on growth rate for the algae population, immobilisation of daphnids and inhibition of rotifer reproduction was observed obtaining EC50 close to 1% of DMSO for all these cases.

摘要

二甲基亚砜 (DMSO) 广泛用作生态毒性生物测定中的载体溶剂。尽管经常使用，但在某些浓度下它可能对生物体有毒。因此，本研究旨在研究 DMSO 对模式生物的影响：微藻Raphidocelis subcapitata、小甲壳动物Daphnia magna和轮虫Brachionus calyciflorus 。DMSO 以 0.031% 至 4% 的浓度应用于生物体。对于R. subcapitata，暴露 72 小时后观察到的最低效应浓度 (LOEC) 为 0.125% DMSO。50%有效浓度（EC ₅₀）为2.138±0.372%。在D. magna 中，对于 1% DMSO，在 24 小时或 48 小时暴露后观察到迁移率的显着差异，即 1.712 ± 0.207% 和 1.167 ± 0.220% DMSO，EC ₅₀分别在 24 小时和 48 小时暴露时观察到。对于B. calyciflorus，无法验证所进行的测试，因为在暴露期结束时在对照条件下存活的动物不足。因此，我们建议在使用B. calyciflorus的测定中避免将 DMSO 作为载体，并在实验溶液中使用最终 DMSO 浓度，R. subcapitata不超过 0.125%， D. magna不超过0.5% 。最后，使用最多 0.01% 的 DMSO 对测试的生物体是安全的。

载体溶剂二甲基亚砜 (DMSO) 对三种水生模式生物的影响进行了研究，即微藻Raphidocelis subcapitata、微型甲壳动物Daphnia magna和轮虫Brachionus calyciflorus 。结果，观察到对藻类种群的生长速率、水蚤的固定和轮虫繁殖的抑制的负面影响，对于所有这些情况，EC50 接近 1% 的 DMSO。