Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
A functionalized magnetic nanoparticle regulated CRISPR-Cas12a sensor for the ultrasensitive detection of alpha-fetoprotein
Analyst ( IF 3.6 ) Pub Date : 2022-05-23 , DOI: 10.1039/d2an00697a Ya Liu 1 , Yan Chen 1 , Yue Zhang 2 , Qi Zhong 1 , Xiaoli Zhu 1 , Qi Wu 1, 3
Analyst ( IF 3.6 ) Pub Date : 2022-05-23 , DOI: 10.1039/d2an00697a Ya Liu 1 , Yan Chen 1 , Yue Zhang 2 , Qi Zhong 1 , Xiaoli Zhu 1 , Qi Wu 1, 3
Affiliation
|
Alpha-fetoprotein (AFP) is an important clinical tumor marker of hepatoblastoma, and the concentration of AFP in serum is closely related to the staging of hepatoblastoma. We report a magnetic bead separation platform based on a switching aptamer triggered hybridization chain reaction (SAT-HCR) and the CRISPR-Cas12a sensor for the in vitro detection of AFP. AFP aptamer, as an easily regulated nucleic acid strand, is responsible for binding to AFP into nucleic acid detection, while HCR-CRISPR-Cas12a, regulated by functionalized magnetic nanoparticles, is responsible for highly specific nucleic acid signal amplification. Under the optimal conditions, the fluorescence intensity was proportional to the concentration of AFP in the range of 0.5–104 ng mL−1 and the limit of detection was 0.170 ng mL−1. In addition, we have successfully applied this biosensor to detect AFP in clinical samples from patients with hepatoblastoma, with greater sensitivity relative to ELISA. Our proposed method showed great potential application in clinical diagnosis and pharmaceutical-related fields with the properties of high sensitivity, low cost and high selectivity.
中文翻译：
用于超灵敏检测甲胎蛋白的功能化磁性纳米颗粒调节 CRISPR-Cas12a 传感器
甲胎蛋白（AFP）是肝母细胞瘤的重要临床肿瘤标志物，血清中AFP的浓度与肝母细胞瘤的分期密切相关。我们报告了一种基于切换适体触发杂交链式反应 (SAT-HCR) 和 CRISPR-Cas12a 传感器的磁珠分离平台，用于体外检测 AFP。AFP适体作为一种易调控的核酸链，负责与AFP结合进入核酸检测，而HCR-CRISPR-Cas12a受功能化磁性纳米粒子调控，负责高度特异性的核酸信号放大。在最佳条件下，荧光强度与AFP浓度成正比，范围为0.5-10 4 ng mL -1检测限为0.170 ng mL -1。此外，我们已成功应用该生物传感器检测肝母细胞瘤患者临床样本中的 AFP，相对于 ELISA 具有更高的灵敏度。我们提出的方法具有高灵敏度、低成本和高选择性的特点，在临床诊断和药物相关领域显示出巨大的应用潜力。
更新日期：2022-05-23
中文翻译：
用于超灵敏检测甲胎蛋白的功能化磁性纳米颗粒调节 CRISPR-Cas12a 传感器
甲胎蛋白（AFP）是肝母细胞瘤的重要临床肿瘤标志物，血清中AFP的浓度与肝母细胞瘤的分期密切相关。我们报告了一种基于切换适体触发杂交链式反应 (SAT-HCR) 和 CRISPR-Cas12a 传感器的磁珠分离平台，用于体外检测 AFP。AFP适体作为一种易调控的核酸链，负责与AFP结合进入核酸检测，而HCR-CRISPR-Cas12a受功能化磁性纳米粒子调控，负责高度特异性的核酸信号放大。在最佳条件下，荧光强度与AFP浓度成正比，范围为0.5-10 4 ng mL -1检测限为0.170 ng mL -1。此外，我们已成功应用该生物传感器检测肝母细胞瘤患者临床样本中的 AFP，相对于 ELISA 具有更高的灵敏度。我们提出的方法具有高灵敏度、低成本和高选择性的特点，在临床诊断和药物相关领域显示出巨大的应用潜力。
京公网安备 11010802027423号