The European Physical Journal E ( IF 1.8 ) Pub Date : 2022-05-23 , DOI: 10.1140/epje/s10189-022-00189-z Stefano Villa 1 , Andrea Palamidessi 2 , Emanuela Frittoli 2 , Giorgio Scita 2, 3 , Roberto Cerbino 4 , Fabio Giavazzi 1
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Abstract
The connection between the properties of a cell tissue and those of the single constituent cells remains to be elucidated. At the purely mechanical level, the degree of rigidity of different cellular components, such as the nucleus and the cytoplasm, modulates the interplay between the cell inner processes and the external environment, while simultaneously mediating the mechanical interactions between neighboring cells. Being able to quantify the correlation between single-cell and tissue properties would improve our mechanobiological understanding of cell tissues. Here we develop a methodology to quantitatively extract a set of structural and motility parameters from the analysis of time-lapse movies of nuclei belonging to jammed and flocking cell monolayers. We then study in detail the correlation between the dynamical state of the tissue and the deformation of the nuclei. We observe that the nuclear deformation rate linearly correlates with the local divergence of the velocity field, which leads to a non-invasive estimate of the elastic modulus of the nucleus relative to the one of the cytoplasm. We also find that nuclei belonging to flocking monolayers, subjected to larger mechanical perturbations, are about two time stiffer than nuclei belonging to dynamically arrested monolayers, in agreement with atomic force microscopy results. Our results demonstrate a non-invasive route to the determination of nuclear relative stiffness for cells in a monolayer.
Graphic abstract
中文翻译：
从显微镜数据的定量分析中无创测量核相对刚度
摘要
细胞组织的特性与单个组成细胞的特性之间的联系仍有待阐明。在纯机械水平上，不同细胞成分（如细胞核和细胞质）的刚性程度调节细胞内部过程与外部环境之间的相互作用，同时调节相邻细胞之间的机械相互作用。能够量化单细胞和组织特性之间的相关性将提高我们对细胞组织的机械生物学理解。在这里，我们开发了一种方法，从属于卡住和植绒细胞单层的细胞核的延时电影分析中定量提取一组结构和运动参数。然后，我们详细研究了组织的动力学状态与细胞核变形之间的相关性。我们观察到核变形率与速度场的局部散度呈线性相关，这导致了核相对于细胞质弹性模量的非侵入性估计。我们还发现，属于植绒单层的原子核，受到较大的机械扰动，比属于动态停滞单层的原子核硬大约两倍，这与原子力显微镜结果一致。我们的结果证明了一种确定单层细胞核相对刚度的非侵入性途径。这导致对细胞核相对于细胞质弹性模量的非侵入性估计。我们还发现，属于植绒单层的原子核，受到较大的机械扰动，比属于动态停滞单层的原子核硬大约两倍，这与原子力显微镜结果一致。我们的结果证明了一种确定单层细胞核相对刚度的非侵入性途径。这导致对细胞核相对于细胞质弹性模量的非侵入性估计。我们还发现，属于植绒单层的原子核，受到较大的机械扰动，比属于动态停滞单层的原子核硬大约两倍，这与原子力显微镜结果一致。我们的结果证明了一种确定单层细胞核相对刚度的非侵入性途径。
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