A resistance gene atlas is an integral component of the breeder’s arsenal in the fight against evolving pathogens. Thanks to high-throughput sequencing, catalogues of resistance genes can be assembled even in crop species with large and polyploid genomes. Here, we report on capture sequencing and assembly of resistance gene homologs in a diversity panel of 907 winter wheat genotypes comprising ex situ genebank accessions and current elite cultivars. In addition, we use accurate long-read sequencing and chromosome conformation capture sequencing to construct a chromosome-scale genome sequence assembly of cv. Attraktion, an elite variety representative of European winter wheat. We illustrate the value of our resource for breeders and geneticists by (i) comparing the resistance gene complements in plant genetic resources and elite varieties and (ii) conducting genome-wide associations scans (GWAS) for the fungal diseases yellow rust and leaf rust using reference-based and reference-free GWAS approaches. The gene content under GWAS peaks was scrutinized in the assembly of cv. Attraktion.

中文翻译：

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抗性基因同源物目录和染色体尺度参考序列支持冬小麦抗性基因定位

抗性基因图谱是育种者对抗进化病原体的武器库中不可或缺的组成部分。由于高通量测序，甚至可以在具有大型多倍体基因组的作物物种中组装抗性基因目录。在这里，我们报告了 907 种冬小麦基因型的多样性面板中抗性基因同源物的捕获测序和组装，包括非原位种质库种质和当前的优良品种。此外，我们使用精确的长读长测序和染色体构象捕获测序来构建 cv 的染色体规模基因组序列组装。Attraktion，代表欧洲冬小麦的优良品种。我们通过 (i) 比较植物遗传资源和优良品种中的抗性基因补体和 (ii) 对真菌病黄锈病和叶锈病进行全基因组关联扫描 (GWAS) 来说明我们的资源对育种者和遗传学家的价值基于参考和无参考的 GWAS 方法。在 cv 的组装中仔细检查了 GWAS 峰下的基因含量。吸引力。