Abnormal endometrial receptivity is a major cause of the failure of embryo transplantation, which may lead to infertility, adverse pregnancy, and neonatal outcomes. While hormonal treatment has dramatically improved the fertility outcomes in women with endometriosis, a substantial unmet need persists in the treatment. In this study, methacrylate gelatin (GelMA) and methacrylate sericin (SerMA) hydrogel with human umbilical cord mesenchymal stem cells (HUMSC) encapsulation was designed for facilitating endometrial regeneration and fertility restoration through in situ injection. The presented GelMA/10%SerMA hydrogel showed appropriate swelling ratio, good mechanical properties, and degradation stability. In vitro cell experiments showed that the prepared hydrogels had excellent biocompatibility and cell encapsulation ability of HUMSC. Further in vivo experiments demonstrated that GelMA/SerMA@HUMSC hydrogel could increase the thickness of endometrium and improve the endometrial interstitial fibrosis. Moreover, regenerated endometrial tissue was more receptive to transfer embryos. Summary, we believed that GelMA/SerMA@HUMSC hydrogel will hold tremendous promise to repair or regenerate damaged endometrium.

中文翻译：

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负载人脐带间充质干细胞的可注射明胶/丝胶水凝胶用于治疗子宫损伤


子宫内膜容受性异常是胚胎移植失败的主要原因，可能导致不孕、不良妊娠和新生儿结局。虽然激素治疗极大地改善了患有子宫内膜异位症的女性的生育结果，但治疗中仍然存在大量未满足的需求。在这项研究中，设计了甲基丙烯酸明胶（GelMA）和甲基丙烯酸丝胶（SerMA）水凝胶与人脐带间充质干细胞（HUMSC）封装，通过原位注射促进子宫内膜再生和生育力恢复。所制备的 GelMA/10%SerMA 水凝胶表现出适当的溶胀比、良好的机械性能和降解稳定性。体外细胞实验表明，所制备的水凝胶具有优异的HUMSC生物相容性和细胞封装能力。进一步的体内实验表明，GelMA/SerMA@HUMSC水凝胶可以增加子宫内膜厚度，改善子宫内膜间质纤维化。此外，再生的子宫内膜组织更容易接受胚胎移植。总之，我们相信 GelMA/SerMA@HUMSC 水凝胶对于修复或再生受损的子宫内膜具有巨大的前景。