Cell aggregates that mimic in vivo cell–cell interactions are promising and powerful tools for tissue engineering. This study isolated a new, easily obtained, population of mesenchymal stem cells (MSCs) from rat hard palates named hard palatal-derived mesenchymal stem cells (PMSCs). The PMSCs were positive for CD90, CD44, and CD29 and negative for CD34, CD45, and CD146. They exhibited clonogenicity, self-renewal, migration, and multipotent differentiation capacities. Furthermore, this study fabricated scaffold-free 3D aggregates using light-controlled cell sheet technology and a serum-free method. PMSC aggregates were successfully constructed with good viability. Transplantation of the PMSC aggregates and the PMSC aggregate-implant complexes significantly enhanced bone formation and implant osseointegration in vivo, respectively. This new cell resource is easy to obtain and provides an alternative strategy for tissue engineering and regenerative medicine.

中文翻译：

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用于骨再生的光控支架和无血清硬腭源性间充质干细胞聚集体


模拟体内细胞-细胞相互作用的细胞聚集体是组织工程的有前途且强大的工具。这项研究从大鼠硬腭中分离出一种新的、容易获得的间充质干细胞 (MSC) 群体，称为硬腭源性间充质干细胞 (PMSC)。 PMSCs CD90、CD44 和 CD29 呈阳性，CD34、CD45 和 CD146 呈阴性。它们表现出克隆形成、自我更新、迁移和多能分化能力。此外，本研究使用光控细胞片层技术和无血清方法制造了无支架 3D 聚集体。 PMSC 聚集体已成功构建，具有良好的生存能力。 PMSC聚集体和PMSC聚集体-种植体复合物的移植分别显着增强了体内骨形成和种植体骨整合。这种新的细胞资源易于获得，为组织工程和再生医学提供了替代策略。