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A hemicyanine-based near-infrared fluorescent probe for vapor-phase hydrazine detection and bioimaging in a complete aqueous media
Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy ( IF 4.3 ) Pub Date : 2022-05-18 , DOI: 10.1016/j.saa.2022.121406
Yang Wang 1 , Xiao-Lei Xue 2 , Qi Zhang 2 , Kun-Peng Wang 2 , Shaojin Chen 2 , Linsheng Tang 3 , Zhi-Qiang Hu 2
Affiliation  

A novel near-infrared fluorescent probe CyOE based on hemicyanine dye containing acetyl as a recognition site is reported. The probe CyOE shows high selectivity and sensitivity (LOD = 82 nM, 2.58 ppb), as well as good water solubility and quantitative detectability of hydrazine in the concentration range of 0–75 μM (R2 = 0.993). Moreover, CyOE has a significant increase in fluorescence at 735 nm with the addition of N2H4, which provides a rapid, colorimetric and gas-phase detection method for N2H4 in both aqueous solution and real water samples. In addition, CyOE is successfully utilized to visualize hydrazine in cells with low cytotoxicity and high cell permeability.



中文翻译:

一种基于半花青的近红外荧光探针,用于在完全水介质中进行气相肼检测和生物成像

报道了一种以乙酰基为识别位​​点的基于半花青染料的新型近红外荧光探针CyOE。CyOE 探针具有高选择性和灵敏度(LOD = 82 nM,2.58 ppb),在 0-75 μM 浓度范围内(R 2  = 0.993)具有良好的水溶性和肼定量检测能力。此外,添加 N 2 H 4后,CyOE 在 735 nm 处的荧光显着增加,这为水溶液和真实水样中的 N 2 H 4提供了一种快速、比色和气相检测方法。此外,CyOE 还成功地用于观察细胞中的肼,具有低细胞毒性和高细胞通透性。

更新日期:2022-05-18
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