Abstract

In the current study, the expression levels of two important lncRNAs, i.e., AK058003 and APOC1P1, in breast tumors were compared with adjacent non-tumor tissues to evaluate their diagnostic potential in a panel of 121 patients. Total RNA was extracted, cDNA was synthesized and expression of AK058003 and APOC1P1 was assessed using qRT-PCR. A significant overexpression and positive correlation between these two lncRNAs were observed in tumor tissues compared to marginal healthy tissues. In conclusion, the examined lncRNAs were overexpressed in tumor tissues, suggesting their significant diagnostic value in breast cancer.

摘要

在目前的研究中，将乳腺肿瘤中两个重要的 lncRNA （即 AK058003 和 APOC1P1）的表达水平与相邻的非肿瘤组织进行比较，以评估它们在一组 121 名患者中的诊断潜力。提取总 RNA，合成 cDNA，并使用 qRT-PCR 评估 AK058003 和 APOC1P1 的表达。与边缘健康组织相比，在肿瘤组织中观察到这两种 lncRNA 之间的显着过表达和正相关。总之，检查的 lncRNA 在肿瘤组织中过表达，表明它们在乳腺癌中具有重要的诊断价值。