Hydrogen gas is a clean fuel with high calorific value. The current study is focused on production of hydrogen gas using Rhodobacter meghalophilus and Thermatoga maritima through anaerobic fermentation. Crude glycerol, by-product from biodiesel plant is used as carbon substrate due to its rich organic composition. Batch experiments have been carried out to study the impact of the inoculum size (1, 2 and 4 mL/L) and crude glycerol (5,10 and 15 mL/L) on the bacterial growth and hydrogen production rates by both the organisms. Inoculum size of 2 mL/L and crude glycerol of 15 mL/L of crude glycerol in medium for fermentation by R. meghalophilus, is found to produce 160 mL/L of gas with hydrogen production rate at 1.163×10-8 m3/kg.s and substrate conversion efficiency of 43.28%. Anaerobic fermentation by T. maritima is found to produce 120 mL/L of gas with 2 mL/L of inoculum and 10 mL/L of crude glycerol with hydrogen production rate of 9.4×10-9 m3/kg.s and substrate conversion efficiency of 23.88%. GC analysis of gas produced by T. maritima and R. meghalophilus shows 25% (v/v) and 19% (v/v) of hydrogen respectively. Thus, R. meghalophilus is found to acclimatize faster and exhibit better hydrogen production rate while T.maritima produces higher yield of hydrogen.

中文翻译：

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利用生物柴油植物粗甘油制取氢气的海嗜热袍菌和嗜红杆菌的比较研究

氢气是一种高热值的清洁燃料。目前的研究重点是通过厌氧发酵利用嗜红杆菌和海马氏菌生产氢气。生物柴油植物的副产品粗甘油由于其丰富的有机成分而被用作碳底物。已经进行了批量实验，以研究接种量（1、2 和 4 mL/L）和粗甘油（5.10 和 15 mL/L）对两种生物的细菌生长和产氢率的影响。R. meghalophilus 发酵培养基中接种量为 2 mL/L 和粗甘油的粗甘油为 15 mL/L，可产生 160 mL/L 的气体，产氢率为 1.163×10-8 m3/kg .s和基板转换效率为43.28%。T. 厌氧发酵 发现maritima产生120 mL/L的气体，2 mL/L的接种物和10 mL/L的粗甘油，产氢率为9.4×10-9 m3/kg.s，底物转化效率为23.88%。T. maritima 和 R. meghalophilus 产生的气体的 GC 分析分别显示 25% (v/v) 和 19% (v/v) 的氢气。因此，发现 R. meghalophilus 适应更快并表现出更好的氢气生产速率，而 T.maritima 产生更高的氢气产量。