Immunomagnetic leukocyte differential in whole blood on an electronic microdevice,Lab on a Chip

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Immunomagnetic leukocyte differential in whole blood on an electronic microdevice
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-05-17 , DOI: 10.1039/d2lc00137c
Ozgun Civelekoglu ₁ , Tevhide Ozkaya-Ahmadov ₁ , A K M Arifuzzman ₁ , Sibel Islak Mutcali ₂ , A Fatih Sarioglu _{1,

3,

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Affiliation

Leukocytes are the frontline defense mechanism of the immune system. Their composition dynamically changes as a response to a foreign body, infection, inflammation, or other malignant behavior occurring within the body. Monitoring the composition of leukocytes, namely leukocyte differential, is a crucial assay periodically performed to diagnose an infection or to assess a person's vulnerability for a health anomaly. Currently, leukocyte differential analysis is performed using hematology analyzers or flow cytometers, both of which are bulky instruments that require trained and certified personnel for operation. In this work, we demonstrate a new technique to obtain leukocyte differentials in a highly portable and integrated microfluidic chip by magnetically analyzing the CD33 expression of leukocytes. When benchmarked against conventional laboratory instruments, our technology demonstrated <5% difference on average for all subtypes. Our results show that hematology testing could be performed beyond the centralized laboratories at a low cost and ultimately provide point-of-care and at-home testing opportunities.

中文翻译：

电子微型设备上全血免疫磁性白细胞差异

白细胞是免疫系统的前线防御机制。它们的组成会随着对异物、感染、炎症或体内发生的其他恶性行为的反应而动态变化。监测白细胞的组成，即白细胞分类，是定期进行的一项重要测定，用于诊断感染或评估一个人对健康异常的脆弱性。目前，白细胞差异分析是使用血液分析仪或流式细胞仪进行的，这两种仪器都是笨重的仪器，需要经过培训和认证的人员才能操作。在这项工作中，我们展示了一种新技术，通过磁性分析白细胞的 CD33 表达，在高度便携和集成的微流控芯片中获得白细胞差异。当以传统实验室仪器为基准时，我们的技术显示所有亚型的平均差异 <5%。我们的研究结果表明，血液学检测可以在集中实验室之外以低成本进行，并最终提供即时和家庭检测机会。

更新日期：2022-05-20

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