Thiophenol (PhSH) is widely used in industry, however, it is extremely harmful to the environment and human health due to its high toxicity. In this work, we developed a new FRET-ICT-based ratiometric fluorescent and colorimetric probe (DMNP) for detecting PhSH. DMNP had an ultrahigh energy transfer efficiency (99.7%) and clear spacing of two emission peaks (133 nm). DMNP achieved a fast response to PhSH and exhibited drastic enhancement (over 2100 folds) of the fluorescence intensity ratio upon addition of PhSH. DMNP showed good linear response in the PhSH concentration ranges of 0.5–13 μM and 17.0–22.0 μM. Meanwhile, DMNP could also be applied to monitor PhSH in a variety of real water samples.

苯硫酚（PhSH）在工业中应用广泛，但由于其高毒性，对环境和人体健康极为有害。在这项工作中，我们开发了一种新的基于 FRET-ICT 的比率荧光和比色探针 ( DMNP )，用于检测 PhSH。DMNP具有超高的能量转移效率（99.7%）和两个发射峰的清晰间距（133 nm）。DMNP实现了对 PhSH 的快速响应，并在添加 PhSH 后显示出荧光强度比的显着增强（超过 2100 倍）。DMNP在 0.5-13 μM 和 17.0-22.0 μM 的 PhSH 浓度范围内显示出良好的线性响应。同时，DMNP也可用于监测各种真实水样中的PhSH。