Effect of addition of Ca2+ to titanium by a hydrothermal method on soft tissue sealing,Microscopy Research and Technique

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Effect of addition of Ca2+ to titanium by a hydrothermal method on soft tissue sealing
Microscopy Research and Technique ( IF 2.0 ) Pub Date : 2022-05-14 , DOI: 10.1002/jemt.24152
Zhendi Fu ₁ , Xuehua Deng ₂ , Xiaodan Fang ₁

Affiliation

The long-term stability of implants requires good peri-implant soft tissue sealing. Calcium ion (Ca²⁺) was loaded onto titanium surface by a hydrothermal method. In vitro, the morphology and composition of titanium surfaces were determined by scanning electron microscopy and energy-dispersive spectroscopy; proliferation of hGF-1 cells was measured by the CCK-8 assay; immunofluorescence staining was done to detect adherent proteins on titanium surface. In vivo, the degree of attachment between the implant and the surrounding soft tissue was measured by horseradish peroxidase (HRP). The percentage of hGF-1 cells adhering in the Ca group was significantly higher (p < .01); the fluorescence of integrin-β1 and F-actin in the Ca group was stronger; Ca group had the shorter length of HRP (p < .01). Ca²⁺ can be added to the surface of titanium by a hydrothermal method and it will be more beneficial for soft tissue early sealing.

中文翻译：

水热法在钛中添加Ca2+对软组织封闭的影响

种植体的长期稳定性需要良好的种植体周围软组织密封。通过水热法将钙离子（Ca ²⁺）负载到钛表面。在体外，采用扫描电子显微镜和能谱仪测定钛表面的形态和组成；通过CCK-8法测量hGF-1细胞的增殖；进行免疫荧光染色以检测钛表面上的粘附蛋白。在体内，植入物与周围软组织之间的附着程度通过辣根过氧化物酶 (HRP) 测量。Ca 组的 hGF-1 细胞粘附率显着升高（p < .01）；Ca组中整合素-β1和F-actin的荧光更强；Ca 组的 HRP 长度较短（p < .01)。Ca ²⁺可以通过水热法添加到钛表面，更有利于软组织的早期封闭。

更新日期：2022-05-14

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