Vascular endothelial growth factor (VEGF₁₆₅) is a signal protein that plays a central role in the regulation of angiogenesis and can stimulate angiogenesis. The development of highly sensitive and selective detection method for VEGF₁₆₅ is very important for disease diagnosis and follow-up treatment monitoring. In this study, an electrochemiluminescence (ECL) aptasensor for VEGF₁₆₅ has been developed based on quench of H₂O₂ toward Ru(bpy)₃²⁺/TPrA ECL system and RecJ_f exonuclease induced target recovery and hybridization chain reaction (HCR) as amplification strategy. The presence of VEGF₁₆₅ makes a large number of glucose oxidase (GOD) fixed on the electrode surface through the double signal amplification strategies. The present of GOD cause the production of a large amount of H₂O₂ near the electrode surface under excess amount of glucose, resulting in the inhibition of the ECL signal of Ru(bpy)₃²⁺/Au nanoparticles (Ru(bpy)₃²⁺/AuNPs) film fixed on the electrode surface. The ECL response of the designed biosensor has a good linear relationship with the logarithm of the concentration of VEGF₁₆₅ in the range of 0.5 pg/mL to 500 ng/mL with a detection limit of 0.2 fg/mL. The VEGF₁₆₅ in serum samples has been detected by the proposed aptasensor with satisfactory results.

血管内皮生长因子（VEGF ₁₆₅）是一种信号蛋白，在血管生成的调节中起核心作用，可刺激血管生成。_{开发高灵敏度、高选择性的VEGF 165}检测方法对疾病诊断和后续治疗监测具有重要意义。在本研究中，基于 H ₂ O ₂对 Ru(bpy) ₃ ²⁺ /TPrA ECL 系统的猝灭和 RecJ _f外切核酸酶诱导的靶点恢复和杂交链式反应 (HCR) ，开发了一种用于 VEGF ₁₆₅的电化学发光 (ECL) 适体传感器。作为放大策略。VEGF _165的存在通过双重信号放大策略使大量葡萄糖氧化酶（GOD）固定在电极表面。GOD的存在导致在过量的葡萄糖下在电极表面附近产生大量H ₂ O _{2 ，​​导致Ru(bpy) 3} ²⁺ /Au纳米粒子(Ru(bpy)的ECL信号受到抑制₃ ²⁺ /AuNPs) 薄膜固定在电极表面。_{所设计的生物传感器的ECL响应与VEGF 165}浓度的对数在0.5 pg/mL至500 ng/mL范围内具有良好的线性关系，检测限为0.2 fg/mL。血管内皮生长因子₁₆₅所提出的适体传感器已检测到血清样品中的含量，结果令人满意。