Apple replant disease (ARD) has been reported in all major apple-growing regions of the world, and abiotic (phenolic compounds including phloridzin) and biological (bacteria, fungi and nematode) factors are important causes of ARD. This study aimed to assess the effect of phloridzin adsorption on biochar pyrolyzed at different temperatures and the effectiveness of biochar addition on the growth of Malus hupehensis Rehd. seedlings under phloridzin stress. Three biochar treatments were considered (BC300, BC400 and BC500), pyrolyzed at three different temperatures (300°C, 400°C and 500°C, respectively). Batch adsorption experiments revealed that BC300 had the maximum adsorption efficiency. The BC300 treatment was then selected for isotherm and kinetic fitting to investigate the influence of pH on the adsorption process and determine the phloridzin adsorption mechanism. The biochar was found to have a single-layer phloridzin adsorption effect, and the adsorption rate was related to the biochar concentration and pyrolysis temperature. A outdoorpot experiment was conducted to analyze the biochar adorption to phloridzin in soil. Phloridzin addition significantly inhibited seedling growth and biochar significantly reduced the soil phloridzin content. BC300 had the best adsorption effect of the three biochar treatments. Compared with the replant soil (CK), biochar (especially BC300) increased the biomass of the seedlings and the antioxidant enzyme activity in the leaves. The soil enzyme activity, organic matter and bacteria numbers increased significantly after biochar addition. The best seedling growth and soil conditions were found for BC300. These results reveal that biochar addition can significantly reduce the phloridzin content and promote seedlings growth, and the effectiveness of the biochar depends on the pyrolysis temperature.

苹果补种病（ARD）在世界所有主要苹果种植区均有报道，非生物（包括根皮苷在内的酚类化合物）和生物（细菌、真菌和线虫）因素是导致ARD的重要原因。本研究旨在评估根皮苷吸附对不同温度热解生物炭的影响以及添加生物炭对湖北海棠生长的有效性。雷德。根皮素胁迫下的幼苗。考虑了三种生物炭处理（BC300、BC400 和 BC500），在三种不同的温度（分别为 300°C、400°C 和 500°C）下热解。批量吸附实验表明，BC300 的吸附效率最高。然后选择BC300处理进行等温线和动力学拟合，以研究pH对吸附过程的影响并确定根皮苷的吸附机理。发现生物炭具有单层根皮苷吸附作用，吸附率与生物炭浓度和热解温度有关。进行了户外盆栽试验，分析了生物炭对土壤中根皮苷的吸附作用。添加根皮苷显着抑制幼苗生长，生物炭显着降低土壤根皮苷含量。BC300在三种生物炭处理中吸附效果最好。与补种土（CK）相比，生物炭（尤其是BC300）增加了幼苗的生物量和叶片中的抗氧化酶活性。添加生物炭后土壤酶活性、有机质和细菌数量显着增加。BC300的幼苗生长和土壤条件最佳。这些结果表明，添加生物炭可以显着降低根皮苷含量并促进幼苗生长，并且生物炭的有效性取决于热解温度。添加生物炭后有机物和细菌数量显着增加。BC300的幼苗生长和土壤条件最佳。这些结果表明，添加生物炭可以显着降低根皮苷含量并促进幼苗生长，并且生物炭的有效性取决于热解温度。添加生物炭后有机物和细菌数量显着增加。BC300的幼苗生长和土壤条件最佳。这些结果表明，添加生物炭可以显着降低根皮苷含量并促进幼苗生长，并且生物炭的有效性取决于热解温度。