Abstract

Three new compounds, 4,5,6,7-tetramethoxy-3-benzoylbenzofuran (1), 4-hydroxy-3,5,6-trimethoxydihydrochalcone-2-O-β-D-glucopyranoside (2) and 2-hydroxy-3,4,5,6-tetramethoxyphenylethyl benzoate (3) along with five known flavonoids were isolated from the dichloromethane fraction of the stems of Fissistigma acuminatissimum Merr.’s ethanol extracts. The compounds were obtained by chromatographic methods and the structure elucidation was completed primarily on the basis of spectroscopic analyses, all of these compounds were isolated from F. acuminatissimum for the first time. All the fractions and compounds were evaluated for their anti-inflammatory activity against lipopolysaccharide (LPS)-stimulated tumor necrosis factor α (TNF-α) production in RAW264.7 cells in vitro. The dichloromethane fraction showed the most potent inhibition(38.2%) at 60 μg/mL, compound 1 (70.2%) and 3 (65.2%) showed significant inhibition at 10 μM.

摘要

三种新化合物，4,5,6,7-tetramethoxy-3-benzoylbenzofuran ( 1 )、4-hydroxy-3,5,6-trimethoxydihydrochalcone-2-O-β-D-glucopyranoside ( 2 ) 和 2-hydroxy- 3,4,5,6-四甲氧基苯甲酸苯乙酯 ( 3 ) 和五种已知的类黄酮是从Fissistigma acuminatissimum Merr. 的乙醇提取物的茎的二氯甲烷部分中分离出来的。这些化合物是通过色谱法获得的，主要在光谱分析的基础上完成结构解析，所有这些化合物都是从F. acuminatissimum中分离得到的首次。评估了所有组分和化合物在体外 RAW264.7 细胞中对脂多糖 (LPS) 刺激的肿瘤坏死因子 α (TNF-α) 产生的抗炎活性。二氯甲烷级分在 60 μg/mL 时表现出最强的抑制作用 (38.2%)，化合物1 (70.2%) 和3 (65.2%) 在 10 μM 时表现出显着抑制作用。