Wood particles of pine required longer treatments of acidic chlorite and dilute alkali to achieve similar proportions of cell separation as of poplar. Chemical extraction and enzymatic digestion studies indicated that rhamnogalacturonan-I (RG-I), mannan and xylan contribute to cell-cell adhesion in pine wood. Mannan associated with lignin in an acidic chlorite fraction and with xylan in a dilute alkali fraction. RG-I and xylan were extracted in both chlorite and dilute alkali in poplar. Scanning electron microscopy and a probe diffusion study using Fluorescence Recovery After Photobleaching showed that lignin, RG-I and mannan initiated cell separation at cell corners and dilute alkali extraction of xylan was required for complete cell separation in pine. Cell-cell separation increased enzymatic saccharification within 24 h by 2-fold in poplar and 3.4-fold in pine. Our results inform a strategy to reduce particle size in woody biomass and provide insights into different molecular bases of cell-cell adhesion.

松木颗粒需要更长的酸性亚氯酸盐和稀碱处理才能达到与杨树相似的细胞分离比例。化学提取和酶消化研究表明，鼠李糖半乳糖醛酸-I (RG-I)、甘露聚糖和木聚糖有助于松木中的细胞-细胞粘附。甘露聚糖与酸性亚氯酸盐馏分中的木质素和稀碱馏分中的木聚糖有关。RG-I 和木聚糖在杨树中的亚氯酸盐和稀碱中均被提取。扫描电子显微镜和使用光漂白后荧光恢复的探针扩散研究表明，木质素、RG-I 和甘露聚糖在细胞角引发细胞分离，而松树细胞完全分离需要木聚糖的稀碱提取。细胞间分离增加酶促杨树 24 小时内糖化 2 倍，松树 3.4 倍。我们的研究结果为降低木质生物质颗粒大小的策略提供了依据，并提供了对细胞-细胞粘附的不同分子基础的见解。