This study aimed to evaluate the effects of glutamine supplementation to soybean meal (SBM) diet in hybrid sturgeon (25.00 ± 1.00 g). Three diets, including fishmeal (FM) diet, SBM diet, and SBMG diet (glutamine addition to SBM diet), were formulated to feed sturgeons for 8 weeks. Growth parameters and muscular crude lipid content were reduced by SBM substitution, and were further increased in SBMG-diet group. In liver morphology detection, moderate lipid droplets accumulation in hepatocytes found in fish fed SBM diet were partially retrieved by glutamine addition. Hepatic gas chromatography–mass spectrometry analysis detected 156 up-regulated metabolites and 15 related KEGG pathways affected by SBM replacement. Glutamine addition resulted in 102 down-regulated metabolites, together with 9 KEGG pathways enriched. In liver transcriptome sequencing analysis, 1074 and 1564 differentially expressed unigenes (DEGs) were detected by SBM/FM and SBMG/SBM pairwise comparisons, respectively. The least number of DEGs (143) was present in pairwise comparison of SBMG /FM. Our results indicated that glutamine addition attenuated the SBM-based negative effect including growth retardation, and muscle proximate composition alteration. Glutamine supplementation alleviated the liver damage caused by SBM substitution possibly through down regulating the elevated levels of metabolites or gene as well as related metabolic pathways.

本研究旨在评估在杂交鲟（25.00 ± 1.00 g）豆粕（SBM）日粮中添加谷氨酰胺的效果。三种饮食，包括鱼粉（FM）饮食、SBM 饮食和 SBMG 饮食（SBM 饮食中添加谷氨酰胺），被配制用于喂养鲟鱼 8 周。SBM 替代降低了生长参数和肌肉粗脂含量，并且在 SBMG 饮食组中进一步增加。在肝脏形态检测中，通过添加谷氨酰胺部分恢复了在喂食 SBM 饮食的鱼中发现的肝细胞中的中度脂滴积累。肝气相色谱-质谱分析检测到 156 种上调代谢物和 15 种受 SBM 替代影响的相关 KEGG 通路。谷氨酰胺添加导致 102 种下调的代谢物，以及 9 种 KEGG 途径富集。在肝脏转录组测序分析中，分别通过 SBM/FM 和 SBMG/SBM 成对比较检测到 1074 和 1564 个差异表达的单基因 (DEG)。在 SBMG / FM 的成对比较中存在最少数量的 DEG（143）。我们的结果表明，谷氨酰胺的添加减弱了基于 SBM 的负面影响，包括生长迟缓和肌肉邻近成分的改变。谷氨酰胺补充剂可能通过下调代谢物或基因的升高水平以及相关代谢途径来减轻由 SBM 替代引起的肝损伤。我们的结果表明，谷氨酰胺的添加减弱了基于 SBM 的负面影响，包括生长迟缓和肌肉邻近成分的改变。谷氨酰胺补充剂可能通过下调代谢物或基因的升高水平以及相关代谢途径来减轻由 SBM 替代引起的肝损伤。我们的研究结果表明，添加谷氨酰胺可减轻基于 SBM 的负面影响，包括生长迟缓和肌肉邻近成分的改变。谷氨酰胺补充剂可能通过下调代谢物或基因的升高水平以及相关代谢途径来减轻由 SBM 替代引起的肝损伤。