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Tuning particle inertial separation in sinusoidal channels by embedding periodic obstacle microstructures
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-05-13 , DOI: 10.1039/d2lc00197g Haotian Cha 1 , Hedieh Fallahi 1 , Yuchen Dai 1 , Sharda Yadav 1 , Samith Hettiarachchi 1 , Antony McNamee 2 , Hongjie An 1 , Nan Xiang 3 , Nam-Trung Nguyen 1 , Jun Zhang 1
Lab on a Chip ( IF 6.1 ) Pub Date : 2022-05-13 , DOI: 10.1039/d2lc00197g Haotian Cha 1 , Hedieh Fallahi 1 , Yuchen Dai 1 , Sharda Yadav 1 , Samith Hettiarachchi 1 , Antony McNamee 2 , Hongjie An 1 , Nan Xiang 3 , Nam-Trung Nguyen 1 , Jun Zhang 1
Affiliation
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Inertial microfluidics functions solely based on the fluid dynamics at relatively high flow speed. Thus, channel geometry is the critical design parameter that contributes to the performance of the device. Four basic channel geometries (i.e., straight, expansion-contraction, spiral and serpentine) have been proposed and extensively studied. To further enhance the performance, innovative channel design through combining two or more geometries is promising. This work explores embedding periodic concave and convex obstacle microstructures in sinusoidal channels and investigates their influence on particle inertial focusing and separation. The concave obstacles could significantly enhance the Dean flow and tune the flow range for particle inertial focusing and separation. Based on this finding, we propose a cascaded device by connecting two sinusoidal channels consecutively for rare cell separation. The concave obstacles are embedded in the second channel to adapt its operational flow rates and enable the functional operation of both channels. Polystyrene beads and breast cancer cells (T47D) spiking in the blood were respectively processed by the proposed device. The results indicate an outstanding separation performance, with 3 to 4 orders of magnitude enhancement in purity for samples with a primary cancer cells ratio of 0.01% and 0.001%, respectively. Embedding microstructures as obstacles brings more flexibility to the design of inertial microfluidic devices, offering a feasible new way to combine two or more serial processing units for high-performance separation.
中文翻译：
通过嵌入周期性障碍物微结构调整正弦通道中的粒子惯性分离
惯性微流体仅基于相对高流速下的流体动力学起作用。因此，通道几何形状是影响器件性能的关键设计参数。四种基本通道几何形状（即、直线、膨胀-收缩、螺旋和蛇形）已被提出并广泛研究。为了进一步提高性能，通过组合两个或更多几何形状的创新通道设计很有前景。这项工作探索了在正弦通道中嵌入周期性凹凸障碍物微结构，并研究它们对粒子惯性聚焦和分离的影响。凹面障碍物可以显着增强 Dean 流动并调整粒子惯性聚焦和分离的流动范围。基于这一发现，我们提出了一种级联装置，通过连续连接两个正弦通道来分离稀有细胞。凹形障碍物嵌入第二个通道中，以适应其运行流速并实现两个通道的功能运行。所提出的装置分别处理了血液中的聚苯乙烯珠和乳腺癌细胞（T47D）。结果表明具有出色的分离性能，对于原发癌细胞比例分别为 0.01% 和 0.001% 的样品，纯度提高了 3 到 4 个数量级。嵌入微结构作为障碍物为惯性微流体装置的设计带来了更大的灵活性，为组合两个或多个串行处理单元以实现高性能分离提供了一种可行的新方法。
更新日期：2022-05-13
中文翻译：
通过嵌入周期性障碍物微结构调整正弦通道中的粒子惯性分离
惯性微流体仅基于相对高流速下的流体动力学起作用。因此，通道几何形状是影响器件性能的关键设计参数。四种基本通道几何形状（即、直线、膨胀-收缩、螺旋和蛇形）已被提出并广泛研究。为了进一步提高性能，通过组合两个或更多几何形状的创新通道设计很有前景。这项工作探索了在正弦通道中嵌入周期性凹凸障碍物微结构，并研究它们对粒子惯性聚焦和分离的影响。凹面障碍物可以显着增强 Dean 流动并调整粒子惯性聚焦和分离的流动范围。基于这一发现，我们提出了一种级联装置，通过连续连接两个正弦通道来分离稀有细胞。凹形障碍物嵌入第二个通道中，以适应其运行流速并实现两个通道的功能运行。所提出的装置分别处理了血液中的聚苯乙烯珠和乳腺癌细胞（T47D）。结果表明具有出色的分离性能，对于原发癌细胞比例分别为 0.01% 和 0.001% 的样品，纯度提高了 3 到 4 个数量级。嵌入微结构作为障碍物为惯性微流体装置的设计带来了更大的灵活性，为组合两个或多个串行处理单元以实现高性能分离提供了一种可行的新方法。
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