Aphids are a major group of crop pests, which directly feed on phloem and indirectly transmit plant virus. The entomopathogenic fungi are essential members of green pest control strategies, and insecticidal RNAi is considered as a new generation of biopesticides. However, the effects of combining entomopathogenic fungus and RNAi-based biopesticides for aphid control are unknown. Here, the responsive genes of Acyrthosiphon pisum were screened from the transcriptomes upon spraying of Beauveria bassiana Bb07 strain (hereafter referred to Bb07). Subsequently, six candidate genes, Ap4714, Ap15782, Ap20844, Ap14741, Ap15105 and Ap16968, were targeted by spray-based RNAi, which produced a range of 20.0, 58.0, 44.0, 27.0, 52.0 and 24.0% gene silencing, respectively. Silencing of single-target (Ap4714, Ap15782, and Ap20844) resulted in increased virulence of Bb07 to Ac. pisum. Importantly, the mortality was increased to 82.1, 76.7, and 60.7% within 7 days to Ac. pisum when double-targets (Ap4714 + Ap15782, Ap4714 + Ap20844 and Ap15782 + Ap20844) were silenced in together with application of Bb07, respectively. This high mortality was also observed in Myzus persicae and Aphis citricidus by co-spray of the combination of dsAp4714 + dsAp15782 together with Bb07. In together, this study demonstrates the potential of a combination of RNAi- and entomopathogenic fungus-based technologies to control aphids, thereby reducing application of chemical pesticides.

蚜虫是作物害虫中的一大类，直接取食韧皮部，间接传播植物病毒。昆虫病原真菌是绿色害虫防治策略的重要成员，杀虫 RNAi 被认为是新一代生物农药。然而，结合昆虫病原真菌和基于 RNAi 的生物农药对蚜虫控制的影响尚不清楚。在这里，在喷洒白僵菌Bb07 菌株（以下简称 Bb07）时，从转录组中筛选出了豌豆蚜的响应基因。随后，六个候选基因Ap4714、Ap15782、Ap20844、Ap14741、Ap15105和Ap16968, 是基于喷雾的 RNAi 的目标, 其产生的基因沉默范围分别为 20.0、58.0、44.0、27.0、52.0 和 24.0%。单目标（Ap4714、Ap15782和Ap20844）的沉默导致 Bb07 对Ac的毒力增加。豌豆。重要的是，死亡率在Ac的 7 天内增加到 82.1%、76.7% 和 60.7% 。双目标时的豌豆（ Ap4714  +  Ap15782，Ap4714  +  Ap20844和Ap15782  +  Ap20844) 分别与 Bb07 的应用一起沉默。通过将 ds Ap4714  + ds Ap15782与 Bb07的组合共喷雾，还在桃蚜和柑橘蚜中观察到这种高死亡率。总之，这项研究证明了 RNAi 和基于昆虫病原真菌的技术相结合控制蚜虫的潜力，从而减少了化学农药的使用。