Effect of Starch Primers on the Fine Structure of Enzymatically Synthesized Glycogen-like Glucan,Journal of Agricultural and Food Chemistry

当前位置： X-MOL 学术 › J. Agric. Food Chem. › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

Effect of Starch Primers on the Fine Structure of Enzymatically Synthesized Glycogen-like Glucan
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-05-12 , DOI: 10.1021/acs.jafc.2c00152
Jialin Liu _{1,

2,

3} , Yanli Wang _{1,

2,

3} , Xiaoxiao Li _{1,

2,

3} , Zhengyu Jin _{1,

2,

3} , Birte Svensson _{4,

5} , Yuxiang Bai _{1,

2,

3,

4}

Affiliation

Glycogen-like glucan (GnG) is a unique hyperbranched polysaccharide nanoparticle which is drawing increasing attention due to its biodegradability and abundant short branches that can be functionalized. Because starch and GnG are both composed of glucose residues and have similar glucosidic bonds, GnG could be fabricated by sucrose phosphorylase, α-glucan phosphorylase, and branching enzymes from starch primers and sucrose. In this study, high-amylose starch, normal starch, and waxy corn starch were used as primers to synthesize GnG, and their impact on the fine structure of GnG was investigated. Structural analysis indicated that with increasing content of amylopectin in the starch primer, the proportion of short chains in GnG decreased, and the degree of β-amylolysis and α-amylolysis was enhanced. Amylose in the primer contributed to a compact and homogeneous structure of GnG, while amylopectin triggered the formation of branch points with a more open distribution. These findings provide a new strategy for regulating the fine structure of GnG.

中文翻译：

淀粉引物对酶法合成糖原样葡聚糖精细结构的影响

糖原样葡聚糖（GnG）是一种独特的超支化多糖纳米颗粒，由于其可生物降解性和丰富的可功能化的短分支而受到越来越多的关注。由于淀粉和 GnG 均由葡萄糖残基组成并具有相似的糖苷键，因此可以通过蔗糖磷酸化酶、α-葡聚糖磷酸化酶以及淀粉引物和蔗糖的分支酶来制造 GnG。本研究以高直链淀粉、普通淀粉和糯玉米淀粉为引物合成GnG，考察了它们对GnG精细结构的影响。结构分析表明，随着淀粉引物中支链淀粉含量的增加，GnG中短链的比例降低，β-淀粉分解和α-淀粉分解的程度增强。引物中的直链淀粉有助于 GnG 的紧凑和均匀的结构，而支链淀粉则引发了分布更开放的分支点的形成。这些发现为调节 GnG 的精细结构提供了新的策略。

更新日期：2022-05-12

点击分享查看原文

点击收藏

阅读更多本刊最新论文本刊介绍/投稿指南11