A rare incidence of multiple bacterial infections was reported in Litopenaeus vannamei grow-out ponds in India. The pathogenic organisms identified are Aeromonas veronii, Bacillus flexus, Kurthia gibsonii, Shewanella decolorationis, Bacillus cereus and Shewanella amazonensis. The infected shrimp showed various clinical and morphological changes such as mucus secretion in the hepatopancreas, bacterial spots in the carapace, red or pink discoloration of abdomen with black stripes and highly melanized body. The Histological studies of the hepatopancreas revealed bacterial plaque in the lumen, inflammatory response, necrosis of connective tissue and lipid droplets. The histology of stomach detected degradation of spongy connective tissue, hypertrophied nucleus and the abdomen showed bacterial plaques in the gaps of the muscle fibre. Histochemically, both gram-positive and gram-negative bacteria's were identified in the hepatopancreas and the presence of mucus in the lumen of hepatopancreatic microtubules was confirmed by Alcian blue staining. The electron microscopic studies of the infected shrimp showed multiple bacterial infections and cellular responses like phagocytosis in the hepatopancreas, using transmission electron microscope and scanning electron microscopic confirmed the presence of bacillus and coccobacillus-shaped bacteria in carapace.

中文翻译：

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太平洋白对虾、南美白对虾多种细菌感染的发生率

据报道，印度南美白对虾养殖池塘发生了罕见的多种细菌感染。鉴定出的病原微生物是气单胞菌、弯曲芽孢杆菌、Kurthia gibsonii、脱色希瓦氏菌、蜡状芽孢杆菌和亚马逊希瓦氏菌。. 被感染的对虾表现出多种临床和形态学变化，如肝胰腺粘液分泌、甲壳细菌斑、腹部红色或粉红色、黑色条纹和高度黑色化的身体。肝胰腺的组织学研究揭示了管腔中的细菌斑块、炎症反应、结缔组织和脂滴的坏死。胃组织学检测海绵状结缔组织退化，核肥大，腹部肌肉纤维间隙有细菌斑块。组织化学上，在肝胰腺中鉴定出革兰氏阳性和革兰氏阴性细菌，并且通过阿尔新蓝染色证实了在肝胰腺微管腔中存在粘液。