Fruit bagging is an effective way of producing pollution-free fruit. However, the micro-environment provided by bagging for fruits could reduce the internal quality for most fruits in various degree. As a novel berry fruit, kiwifruit (Actinidia eriantha) has great potential for development. We found a change in fruit quality after fruit shading by bagging (FSB). In order to explore the regulatory mechanism of FSB on fruit quality, we used off-target metabolomics and transcriptomics to identify differential metabolites and differential genes, and focused on the molecular mechanism of the decrease in AsA content. We found that the contents of soluble solids (SSC), soluble sugar (SS), ascorbic acid (AsA), chlorophyll and carotenoid in the FSB fruit were significantly reduced. Through the non-targeted metabolome, a total of 160 metabolites showed significant differences in fruit tissue. There were 3,200 differentially expressed genes (DEGs) between FSB and control by RNA-Seq and enriched in 118 KEGG metabolic pathways. The decrease of AsA content after FSB was mainly due to the decrease of DHAR gene, MDHAR gene, APX gene and the increase of MDHA metabolite during the AsA-GSH cycle process. The decrease of GalUR gene in d-galacturonic acid pathway and the decrease of GulLO gene and Myo-inositol metabolite in Myo-inositol pathway were the secondary reasons for the decrease of AsA content. This study would provide novel insights into the effects of FSB on changes in the intrinsic and appearance quality of A. eriantha fruit.

水果套袋是生产无公害水果的有效方式。但套袋为果实提供的微环境不同程度地降低了大部分果实的内在品质。作为一种新型浆果类水果，猕猴桃（Actindia eriantha) 具有很大的发展潜力。我们发现套袋遮光 (FSB) 后水果质量发生了变化。为了探索FSB对果实品质的调控机制，我们利用脱靶代谢组学和转录组学对差异代谢物和差异基因进行鉴定，重点研究了AsA含量降低的分子机制。我们发现FSB果实中可溶性固形物（SSC）、可溶性糖（SS）、抗坏血酸（AsA）、叶绿素和类胡萝卜素的含量显着降低。通过非靶向代谢组，共有 160 种代谢物在果实组织中表现出显着差异。通过 RNA-Seq 在 FSB 和对照之间有 3,200 个差异表达基因 (DEG)，并富集了 118 个 KEGG 代谢途径。FSB后AsA含量下降主要是由于DHAR基因、MDHAR基因、APX基因与AsA-GSH循环过程中MDHA代谢物的增加。d-半乳糖醛酸途径中GalUR基因的减少和Myo-肌醇途径中GulLO基因和Myo-肌醇代谢物的减少是AsA含量降低的次要原因。这项研究将为 FSB 对A. eriantha果实内在和外观质量变化的影响提供新的见解。