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In vitro electrochemical measurement of serotonin release in the human jejunum mucosa using a diamond microelectrode
Analyst ( IF 3.6 ) Pub Date : 2022-05-11 , DOI: 10.1039/d2an00487a Marion France 1 , James J Galligan 2, 3 , Greg M Swain 1, 3
Analyst ( IF 3.6 ) Pub Date : 2022-05-11 , DOI: 10.1039/d2an00487a Marion France 1 , James J Galligan 2, 3 , Greg M Swain 1, 3
Affiliation
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We report herein on the use of a boron-doped diamond microelectrode (DME) to record oxidation currents in vitro associated with the release of serotonin from enterochromaffin cells in the epithelium of the human intestinal mucosa. Continuous amperometric measurements were made as a function of distance (ln current vs. distance) from the tissue surface in human jejunum specimens. The results demonstrate the capabilities of the DME for the stable and reproducible detection of serotonin in the complex environment of the human tissue. Serotonin release was evoked by the shearing force of a continuously flowing Krebs buffer solution at 36 °C with the tissue pinned down in a flow bath. Reproducible currents with distance were recorded for serotonin oxidation. Increased oxidation currents were observed in the presence of the selective serotonin reuptake inhibitor, fluoxetine, indicating that a significant fraction of the amperometric current recorded is attributable to serotonin oxidation. The nominal reciprocal slope, |slope−1|, of the ln current vs. distance plots increased from 270 ± 25 μm−1 in Krebs buffer (N = 3) to 471 ± 65 μm−1 during fluoxetine addition (N = 3), reflective of a reduced rate of reuptake in the presence of the SERT antagonist. The paper reports on the characterization of the diamond microelectrodes and the in vitro electrochemical measurement data.
中文翻译：
使用金刚石微电极体外电化学测量人空肠粘膜中血清素的释放
我们在此报告了使用掺硼金刚石微电极 (DME) 来记录与人肠粘膜上皮细胞中肠嗜铬细胞释放血清素相关的体外氧化电流。连续安培测量作为距离的函数进行（ln 电流对电流）。距离）从人空肠标本的组织表面。结果证明了 DME 在人体组织的复杂环境中稳定和可重复地检测血清素的能力。血清素释放是由连续流动的 Krebs 缓冲溶液在 36°C 下的剪切力引起的，组织固定在流动浴中。记录了血清素氧化随距离的可重现电流。在选择性血清素再摄取抑制剂氟西汀存在下观察到氧化电流增加，表明记录的安培电流的很大一部分可归因于血清素氧化。ln 电流与距离图的标称倒数斜率 |slope −1 |从 270 ± 25 μm 增加Krebs 缓冲液中的-1 ( N = 3) 至添加氟西汀期间的471 ± 65 μm -1 ( N = 3)，反映了在存在 SERT 拮抗剂的情况下再摄取率降低。该论文报告了金刚石微电极的表征和体外电化学测量数据。
更新日期：2022-05-11
中文翻译：
使用金刚石微电极体外电化学测量人空肠粘膜中血清素的释放
我们在此报告了使用掺硼金刚石微电极 (DME) 来记录与人肠粘膜上皮细胞中肠嗜铬细胞释放血清素相关的体外氧化电流。连续安培测量作为距离的函数进行（ln 电流对电流）。距离）从人空肠标本的组织表面。结果证明了 DME 在人体组织的复杂环境中稳定和可重复地检测血清素的能力。血清素释放是由连续流动的 Krebs 缓冲溶液在 36°C 下的剪切力引起的，组织固定在流动浴中。记录了血清素氧化随距离的可重现电流。在选择性血清素再摄取抑制剂氟西汀存在下观察到氧化电流增加，表明记录的安培电流的很大一部分可归因于血清素氧化。ln 电流与距离图的标称倒数斜率 |slope −1 |从 270 ± 25 μm 增加Krebs 缓冲液中的-1 ( N = 3) 至添加氟西汀期间的471 ± 65 μm -1 ( N = 3)，反映了在存在 SERT 拮抗剂的情况下再摄取率降低。该论文报告了金刚石微电极的表征和体外电化学测量数据。
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