Implementations of wearable microneedle-based arrays of sensors for the monitoring of multiple biomarkers in interstitial fluid have lacked system integration and evidence of robust analytical performance. Here we report the development and testing of a fully integrated wearable array of microneedles for the wireless and continuous real-time sensing of two metabolites (lactate and glucose, or alcohol and glucose) in the interstitial fluid of volunteers performing common daily activities. The device works with a custom smartphone app for data capture and visualization, comprises reusable electronics and a disposable microneedle array, and is optimized for system integration, cost-effective fabrication via advanced micromachining, easier assembly, biocompatibility, pain-free skin penetration and enhanced sensitivity. Single-analyte and dual-analyte measurements correlated well with the corresponding gold-standard measurements in blood or breath. Further validation of the technology in large populations with concurrent validation of sensor readouts through centralized laboratory tests should determine the robustness and utility of real-time simultaneous monitoring of several biomarkers in interstitial fluid.

用于监测间质液中多种生物标志物的可穿戴式微针传感器阵列的实现缺乏系统集成和稳健分析性能的证据。在这里，我们报告了完全集成的可穿戴微针阵列的开发和测试，用于无线和连续实时感测执行日常活动的志愿者间质液中的两种代谢物（乳酸和葡萄糖，或酒精和葡萄糖）。该设备与自定义智能手机应用程序配合使用以进行数据捕获和可视化，包括可重复使用的电子设备和一次性微针阵列，并针对系统集成进行了优化，通过先进的微机械加工具有成本效益的制造，更容易组装，生物相容性，无痛皮肤渗透和增强灵敏度。单分析物和双分析物测量与血液或呼吸中相应的金标准测量密切相关。在大量人群中进一步验证该技术，并通过集中实验室测试同时验证传感器读数，应确定实时同时监测间质液中多种生物标志物的稳健性和实用性。