Gel staining is a crucial step that allows the visualisation of proteins separated through SDS-PAGE. Colloidal Coomassie Brilliant Blue-G (CBB-G) staining is among the commonly used visualisation methods due to several factors such as compatibility with mass spectrometry (MS) analysis, sensitivity, reproducibility, and simplicity of the staining process. However, the standard colloidal CBB-G staining has a drawback: the resolution of protein bands is compromised because of diffusion of proteins during the washing step. A modification to an established colloidal CBB-G staining method, which greatly increases the resolution of protein bands, is described. The addition of a fixation step, which prevents the diffusion of proteins during the washing step, is shown to increase protein band resolution. The fixation step is fast, flexible, and also retains all the advantages of the standard colloidal CBB-G staining methods. As there are no drawbacks, incorporating this fixation step into the standard colloidal CBB-G staining is an easy way to improve protein visualisation in SDS-PAGE.

中文翻译：

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通过凝胶固定改善胶体 CBB-G 染色，实现更高分辨率的蛋白质条带可视化

凝胶染色是一个关键步骤，它允许通过 SDS-PAGE 分离蛋白质的可视化。胶体考马斯亮蓝-G (CBB-G) 染色是常用的可视化方法之一，因为它与质谱 (MS) 分析的兼容性、灵敏度、重现性和染色过程的简单性等几个因素。然而，标准胶体 CBB-G 染色有一个缺点：由于蛋白质在洗涤步骤中的扩散，蛋白质条带的分辨率受到影响。描述了对已建立的胶体 CBB-G 染色方法的修改，该方法大大提高了蛋白质条带的分辨率。添加固定步骤可防止蛋白质在洗涤步骤中扩散，可提高蛋白质条带分辨率。固定步骤快速、灵活、并且还保留了标准胶体 CBB-G 染色方法的所有优点。由于没有缺点，将此固定步骤纳入标准胶体 CBB-G 染色是改善 SDS-PAGE 中蛋白质可视化的简单方法。