The purpose of this study was to investigate the effect of an activation solution (AS) (hatchery water (pH 7.9 and 4 mOsm/kg), Perchec solution (5 mM KCl, 45 mM NaCl, 30 mM Tris-HCl; pH 8.0 and 130 mOsm/kg) and Cheng solution (40 mM NaCl, 20 mM Tris; pH 8.0 and 100 mOsm/kg) on aging sperm of common carp (Cyprinus carpio) after storage in a carp artificial seminal plasma (carp ASP: 2 mM CaCl₂, 1 mM MgSO₄, 40 mM KCl, 110 mM NaCl, 20 mM Tris; pH 7.5 and 310 mOsm/kg) for 7 days without antibiotics at 4 °C. Fresh milt and undiluted aging milt were used as the control groups. Milt (5 ml) was collected and diluted 1:1 in a carp ASP in 50 ml containers and stored at 4 °C under aerobic conditions. The highest sperm motility was found 45% and 36% at 15 s and 45 s post sperm activation (PSA) in hatchery water. Additionally, the curvilinear velocity (VCL, μm/s) and straight-line velocity (VSL, µm/s) of sperm were the highest in hatchery water and the solution with 5 mM KCl, 45 mM NaCl, 30 mM Tris and pH 8.0, which were above 122 µm/s and 87 µm/s at 15 PSA. Sperm concentration in aging sperm of common carp is known to decrease rapidly, so prior to fertilization, sperm concentration was determined and accurately set to 200,000 sperm per egg. The fertilization of eggs and hatching rate after 7 days of sperm storage in carp ASP were 85–87% and 78–81% in both ASs of hatchery water and Perchec solution. Less than 1% sperm motility, fertilization and hatching rate were observed in undiluted sperm (control). In conclusion, the fertilization ability of aging common carp sperm in carp ASP within different ASs was confirmed for practical use in aquaculture.

本研究的目的是研究活化溶液 (AS)（孵化水（pH 7.9 和 4 mOsm/kg）、Perchec 溶液（5 mM KCl、45 mM NaCl、30 mM Tris-HCl；pH 8.0 和130 mOsm/kg) 和 Cheng 溶液 (40 mM NaCl, 20 mM Tris; pH 8.0 和 100 mOsm/kg) 对储存在鲤鱼人工精浆 (鲤鱼 ASP: 2 mM CaCl)的鲤鱼 ( Cyprinus carpio ) 老化精子₂ , 1 毫米硫酸镁₄、40 mM KCl、110 mM NaCl、20 mM Tris；pH 7.5 和 310 mOsm/kg) 在 4 °C 下不使用抗生素 7 天。新鲜鱼精和未稀释的老化鱼精作为对照组。在 50 ml 容器中的鲤鱼 ASP 中收集和稀释 1:1 的鱼糜（5 ml），并在有氧条件下于 4 °C 下储存。在孵化场水中的精子激活 (PSA) 后 15 秒和 45 秒，发现最高的精子活力分别为 45% 和 36%。此外，精子的曲线速度（VCL，μm/s）和直线速度（VSL，μm/s）在孵化用水和含有 5 mM KCl、45 mM NaCl、30 mM Tris 和 pH 8.0 的溶液中最高，在 15 PSA 时高于 122 µm/s 和 87 µm/s。众所周知，鲤鱼衰老精子中的精子浓度会迅速下降，因此在受精之前，要确定精子浓度并准确设置为每个卵子 200,000 个精子。在孵化水和 Perchec 溶液的 ASs 中，鲤鱼 ASP 精子储存 7 天后的卵受精率和孵化率分别为 85-87% 和 78-81%。在未稀释的精子（对照）中观察到小于 1% 的精子活力、受精和孵化率。总之，不同AS内鲤鱼ASP中老化的鲤鱼精子的受精能力被证实可用于水产养殖。