An effective method based on tissue-smashing extraction assisted aqueous two-phase system (TSE-ATPS) was developed for extracting Lycium barbarum L. fruits polysaccharides (LBPs). The ethanol/salt aqueous two-phase system (ATPS) composed of three different salts was evaluated, from which the system of 17.86% (NH₄)₂SO₄ (w/w) and 28.86% ethanol (w/w) was chosen. The conditions were as follows: solid-to-solvent ratio 1:30 (m/v), tissue-smashing power 8000 rpm (8 krpm), extraction time 4 min and the extraction yield of LBPs was 24.79 mg/g. Compared with hot water extraction (HWE) and ultrasound-assisted extraction (UAE), TSE-ATPS ((NH₄)₂SO₄/ethanol) improved the extraction yield of LBPs. LBPs extracted using various methods have different chemical structures and monosaccharide compositions. Superior antioxidant activity was demonstrated when the ATPS was composed of K₂HPO₄/ethanol and NaH₂PO₄/ethanol. Therefore, as an innovative, promising, and rapid crushing extraction technology, TSE-ATPS provides a better choice for extraction and separation of polysaccharides from natural products.

建立了一种基于组织粉碎辅助水相两相系统（TSE-ATPS）提取枸杞多糖（LBPs）的有效方法。评价了由三种不同盐组成的乙醇/盐水溶液两相体系（ATPS），从中选择了17.86%（NH ₄）₂ SO ₄（w/w）和28.86%乙醇（w/w）的体系. 条件如下：固溶比1:30（m/v），组织粉碎力8000 rpm（8 krpm），提取时间4 min，LBPs的提取率为24.79 mg/g。与热水提取（HWE）和超声辅助提取（UAE）相比，TSE-ATPS ((NH ₄ ) ₂ SO ₄/乙醇）提高了LBPs的提取率。使用各种方法提取的 LBPs 具有不同的化学结构和单糖组成。当ATPS由K ₂ HPO ₄ /乙醇和NaH ₂ PO _{4 /乙醇组成时，表现出}优异的抗氧化活性。因此，TSE-ATPS作为一种创新、有前景的快速粉碎提取技术，为天然产物中多糖的提取分离提供了更好的选择。