Oregano essential oil (OEO) has great antioxidant and antifungal potential as a natural bioinhibitor, which is limited by its volatility and instability. In this study, OEO was loaded onto mesoporous nanosilica (MNS) to prolong the efficacy of biological inhibitors and then oxidized esterified tapioca starch (OETS) was used as a film-forming matrix to develop bio-nanocomposites for packaging by the casting method. Transmission electron microscopy (TEM), fourier transform infrared (FT-IR), and thermogravimetric analysis (TGA) were used to analyze the loading effect of nanoparticles. Then, the microstructure, physical, barrier, antioxidant and antifungal properties of the active films containing OEO–MNS were evaluated and compared with those of the OETS-based film. The results showed that the presence of OEO–MNS endowed the film with antioxidant, antifungal, and ultraviolet-blocking properties, as well as improved tensile strength (TS) and water resistance. Specifically, the best performance was obtained for the OETS-based film containing 0.9% OEO–MNS with a uniform nanoparticle distribution. Meanwhile, the TS of the active films reached up to 6.97 MPa, which was about 236% higher than that of the pure OETS-based films. In addition, the film incorporating 1.5% OEO–MNS had the highest antioxidant capacity and excellent antifungal activity. The prepared multifunctional nanocomposite films containing essential oil have promising applications in the field of active packaging.

牛至精油(OEO) 作为天然生物抑制剂具有很强的抗氧化和抗真菌潜力，但受限于其挥发性和不稳定性。在这项研究中，将OEO负载到介孔纳米二氧化硅（MNS）上以延长生物抑制剂的功效，然后使用氧化酯化木薯淀粉（OETS）作为成膜基质，通过流延法开发用于包装的生物纳米复合材料。透射电子显微镜(TEM)、傅里叶变换红外 (FT-IR) 和热重分析 (TGA) 用于分析纳米粒子的负载效果。然后，对含有OEO-MNS的活性薄膜的微观结构、物理、阻隔、抗氧化和抗真菌性能进行了评估，并与OETS基薄膜进行了比较。结果表明，OEO-MNS的存在赋予了薄膜抗氧化、抗真菌和紫外线阻挡性能，并提高了拉伸强度（TS）和耐水性。具体来说，含有 0.9% OEO-MNS 且纳米粒子分布均匀的 OETS 基薄膜获得了最佳性能。同时，活性薄膜的 TS 高达 6.97 MPa，比纯 OETS 基薄膜高约 236%。此外，加入 1.5% OEO-MNS 的薄膜具有最高的抗氧化能力和优异的抗真菌活性。制备的含有精油的多功能纳米复合薄膜在活性包装领域具有广阔的应用前景。