In this work, fungal mushroom-derived carboxymethyl chitosan-polydopamine hydrogels (FCMCS-PDA) with multifunctionality (tissue adhesive, hemostasis, self-healing, and antibacterial properties) were developed for wound dressing applications. The hydrogel is obtained through dynamic Schiff base cross-linking and hydrogen bonds between FCMCS-PDA and covalently cross-linked polyacrylamide (PAM) networks. The FCMCS-PDA-PAM hydrogels have a good swelling ratio, biodegradable properties, excellent mechanical properties, and a highly interconnected porous structure with PDA microfibrils. Interestingly, the PDA microfibrils were formed along with FCMCS fibers in the hydrogel networks, which has a high impact on the biological performance of hydrogels. The maximum adhesion strength of the hydrogel to porcine skin was achieved at about 29.6 ± 2.9 kPa. The hydrogel had good self-healing and recoverable properties. The PDA-containing hydrogels show good antibacterial properties on Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus) bacteria. Moreover, the adhesive hydrogels depicted good viability and attachment of skin fibroblasts and keratinocyte cells. Importantly, FCMCS and PDA combined resulted in fast blood coagulation within 60 s. Hence, the adhesive hydrogel with multifunctionality has excellent potential as a wound dressing material for infected wounds.

中文翻译：

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真菌衍生羧甲基壳聚糖-聚多巴胺水凝胶的组织粘附、自愈、生物相容、止血和抗菌特性

在这项工作中，开发了具有多功能（组织粘合剂、止血、自愈和抗菌特性）的真菌蘑菇衍生的羧甲基壳聚糖-聚多巴胺水凝胶 (FCMCS-PDA)，用于伤口敷料应用。水凝胶是通过 FCMCS-PDA 和共价交联聚丙烯酰胺 (PAM) 网络之间的动态席夫碱交联和氢键获得的。FCMCS-PDA-PAM水凝胶具有良好的溶胀率、可生物降解性能、优异的机械性能以及与PDA微纤维高度互连的多孔结构。有趣的是，PDA 微纤维与 FCMCS 纤维一起在水凝胶网络中形成，这对水凝胶的生物学性能有很大影响。水凝胶对猪皮肤的最大粘附强度约为 29.6 ± 2.9 kPa。该水凝胶具有良好的自愈性和可恢复性。含 PDA 的水凝胶在大肠杆菌（E.coli）和金黄色葡萄球菌（S. aureus）细菌。此外，粘性水凝胶表现出良好的活力和皮肤成纤维细胞和角质形成细胞的附着力。重要的是，FCMCS 和 PDA 结合可在 60 秒内实现快速凝血。因此，具有多功能的粘性水凝胶具有作为感染伤口的伤口敷料材料的良好潜力。