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Simple and Rapid Method for the Quantitation of Trace-Level 3-Alkyl-2-methoxypyrazines in Fragrant Vegetable Oils by Gas Chromatography–Tandem Mass Spectrometry
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-05-10 , DOI: 10.1021/acs.jafc.1c07380 Chuan Zhou 1 , Ziyan Xu 1 , Haiming Shi 1 , Ge Zhang 1 , Wenming Cao 1 , Xuebing Xu 1
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-05-10 , DOI: 10.1021/acs.jafc.1c07380 Chuan Zhou 1 , Ziyan Xu 1 , Haiming Shi 1 , Ge Zhang 1 , Wenming Cao 1 , Xuebing Xu 1
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3-Alkyl-2-methoxypyrazines (MPs) are characteristic aroma compounds found in fragrant vegetable oils, a type of specially processed oils with enhanced flavor. MP contents in these oils are usually at trace level, which makes their quantification a big challenge. In this work, we describe an optimized approach with a double-step acid/alkali extraction for the analysis of such compounds, namely, 3-isopropyl-2-methoxypyrazine, 3-isobutyl-2-methoxypyrazine, and 3-sec-butyl-2-methoxypyrazine, in those fragrant oils using gas chromatography–tandem mass spectrometry (GC–MS/MS). The sample preparation conditions including selections and percentages of acids, alkalis, and extraction solvents, as well as the stability of MPs, were optimized and examined. Method validation was conducted with a good linearity (r2 > 0.999), and average recoveries between 93.9 and 109.3% were achieved. The limit of detection ranged from 0.2 to 0.4 μg/kg, and the relative standard deviations varied from 0.4 to 12.2% for samples spiked with the MPs at different concentrations. Overall, the method satisfactorily meets the requirements for the measurement of trace-level MPs in the fragrant vegetable oils via odor activity value calculation, and the results indicate that the improved acid/alkali extraction method is suitable for the routine analysis of MPs in those vegetable oils.
中文翻译：
气相色谱-串联质谱法测定芳香植物油中痕量 3-烷基-2-甲氧基吡嗪的简单快速方法
3-Alkyl-2-methoxypyrazines (MPs) 是芳香植物油中的特征芳香化合物，植物油是一种风味增强的特殊加工油。这些油中的 MP 含量通常处于痕量水平，这使得它们的量化成为一个巨大的挑战。在这项工作中，我们描述了一种采用双步酸/碱萃取的优化方法，用于分析此类化合物，即 3-异丙基-2-甲氧基吡嗪、3-异丁基-2-甲氧基吡嗪和 3-仲丁基- 2-甲氧基吡嗪，在那些芳香油中使用气相色谱-串联质谱 (GC-MS/MS)。对酸、碱和萃取溶剂的选择和比例以及 MPs 的稳定性等样品制备条件进行了优化和检验。方法验证以良好的线性进行（r 2 > 0.999)，平均回收率在 93.9% 和 109.3% 之间。加标不同浓度 MPs 的样品的检测限为 0.2 至 0.4 μg/kg，相对标准偏差为 0.4% 至 12.2%。总体而言，该方法通过气味活性值计算满足了对芳香植物油中微量MPs测定的要求，结果表明改进后的酸碱提取法适用于植物油中MPs的常规分析。油。
更新日期：2022-05-10
中文翻译：
气相色谱-串联质谱法测定芳香植物油中痕量 3-烷基-2-甲氧基吡嗪的简单快速方法
3-Alkyl-2-methoxypyrazines (MPs) 是芳香植物油中的特征芳香化合物，植物油是一种风味增强的特殊加工油。这些油中的 MP 含量通常处于痕量水平，这使得它们的量化成为一个巨大的挑战。在这项工作中，我们描述了一种采用双步酸/碱萃取的优化方法，用于分析此类化合物，即 3-异丙基-2-甲氧基吡嗪、3-异丁基-2-甲氧基吡嗪和 3-仲丁基- 2-甲氧基吡嗪，在那些芳香油中使用气相色谱-串联质谱 (GC-MS/MS)。对酸、碱和萃取溶剂的选择和比例以及 MPs 的稳定性等样品制备条件进行了优化和检验。方法验证以良好的线性进行（r 2 > 0.999)，平均回收率在 93.9% 和 109.3% 之间。加标不同浓度 MPs 的样品的检测限为 0.2 至 0.4 μg/kg，相对标准偏差为 0.4% 至 12.2%。总体而言，该方法通过气味活性值计算满足了对芳香植物油中微量MPs测定的要求，结果表明改进后的酸碱提取法适用于植物油中MPs的常规分析。油。
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