Point-of-Care Blood Coagulation Assay Based on Dynamic Monitoring of Blood Viscosity Using Droplet Microfluidics,ACS Sensors

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Point-of-Care Blood Coagulation Assay Based on Dynamic Monitoring of Blood Viscosity Using Droplet Microfluidics
ACS Sensors ( IF 8.2 ) Pub Date : 2022-05-10 , DOI: 10.1021/acssensors.1c02360
Linzhe Chen _{1,

2} , Donghao Li _{1,

2} , Xinyu Liu _{1,

2,

3} , Yihan Xie _{1,

2} , Jieying Shan _{1,

2} , Haofan Huang _{1,

2} , Xiaxia Yu _{1,

2} , Yudan Chen ₄ , Weidong Zheng ₄ , Zida Li _{1,

2}

Affiliation

Monitoring of the coagulation function has applications in many clinical settings. Routine coagulation assays in the clinic are sample-consuming and slow in turnaround. Microfluidics provides the opportunity to develop coagulation assays that are applicable in point-of-care settings, but reported works required bulky sample pumping units or costly data acquisition instruments. In this work, we developed a microfluidic coagulation assay with a simple setup and easy operation. The device continuously generated droplets of blood sample and buffer mixture and reported the temporal development of blood viscosity during coagulation based on the color appearance of the resultant droplets. We characterized the relationship between blood viscosity and color appearance of the droplets and performed experiments to validate the assay results. In addition, we developed a prototype analyzer equipped with simple fluid pumping and economical imaging module and obtained similar assay measurements. This assay showed great potential to be developed into a point-of-care coagulation test with practical impact.

中文翻译：

基于使用液滴微流体动态监测血液粘度的即时凝血测定

凝血功能的监测在许多临床环境中都有应用。临床中的常规凝血测定耗费大量样本且周转缓慢。微流体技术提供了开发适用于床旁环境的凝血测定的机会，但报告的工作需要笨重的样本泵送装置或昂贵的数据采集仪器。在这项工作中，我们开发了一种设置简单、操作方便的微流控凝血测定。该装置连续产生血样和缓冲液混合物的液滴，并根据所得液滴的颜色外观报告凝血过程中血液粘度的时间变化。我们描述了血液粘度与液滴颜色外观之间的关系，并进行了实验以验证测定结果。此外，我们开发了一种原型分析仪，配备了简单的流体泵和经济的成像模块，并获得了类似的测定测量结果。该测定显示出发展成为具有实际影响的床旁凝血试验的巨大潜力。

更新日期：2022-05-10

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