Abstract

This study evaluated the effects of micrometric or nano-sized sodium hexametaphosphate (HMPnano), combined or not with fluoride (NaF, 1100 ppm), on dual-species biofilms of Streptococcus mutans and Candida albicans. Biofilms were treated with solutions containing the polyphosphates at 0.5% or 1.0%, with/without fluoride (F), in addition to positive and negative controls. Biofilms were analysed by colony-forming units (CFU) counting, metabolic activity, production of biomass, composition of extracellular matrix, and structure. 1% HMPnano + F led to the lowest S. mutans CFU, while C. albicans CFU counts were not affected by any solution. 1% HMPnano led to the lowest metabolic activity, except for 1% HMPnano + F. All solutions promoted reductions in biofilm biomass compared to controls. Also, 1% HMPnano + F promoted the lowest concentrations of carbohydrates in the biofilm matrix, besides substantially affecting biofilms’ structure. In conclusion, HMPnano and F promoted higher antibiofilm effects compared with its micrometric counterpart for most of the parameters assessed.

摘要

本研究评估了微米或纳米六偏磷酸钠 (HMPnano) 与氟化物 (NaF, 1100 ppm) 结合或不结合对变形链球菌和白色念珠菌双物种生物膜的影响。除了阳性和阴性对照外，生物膜还用含有 0.5% 或 1.0% 多磷酸盐、有/无氟化物 (F) 的溶液处理。通过菌落形成单位 (CFU) 计数、代谢活性、生物质的产生、细胞外基质的组成和结构来分析生物膜。1% HMPnano + F 导致变形链球菌CFU 最低，而白色念珠菌CFU 计数不受任何解决方案的影响。1% HMPnano 导致最低的代谢活性，除了 1% HMPnano + F。与对照相比，所有解决方案都促进了生物膜生物量的减少。此外，1% HMPnano + F 促进了生物膜基质中碳水化合物的最低浓度，除了显着影响生物膜的结构。总之，对于大多数评估的参数，HMPnano 和 F 与其微米对应物相比，促进了更高的抗生物膜效应。