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Genetic and reproductive mode analyses of a golden-back mutant of crucian carp from a rice-fish integrated farming system
Aquaculture Reports ( IF 3.2 ) Pub Date : 2022-05-09 , DOI: 10.1016/j.aqrep.2022.101146
Xian-bo Zhang 1 , Jian-jun Fu 2, 3 , Ming-kun Luo 2, 3 , Zhuo-ma Cai-rang 1 , Qian-wen Min 1 , Jin-li Hu 1 , Sheng Zeng 1 , Tian-xun Luo 1 , Wen-bin Zhu 2, 3 , Ting Liu 1 , Zai-jie Dong 2, 3
Affiliation  

Traditional agricultural systems demonstrated farmers' wisdom in farming, while extending many new agricultural ecosystems and breeding a lot of new and interesting organisms. This study introduced the discovery of an indigenous golden-back crucian carp (GBCrC; Carassius gibelio) from the Dong's Rice-Fish-Duck System, one of the Globally Important Agricultural Heritage Systems in the world, by exploring and analyzing its external morphological characteristics, distribution, paddy field selection, reproductive mode, genetic diversity and origin. It was found that about 27.33% of 150 surveyed paddy fields in 6 villages, characterized by exchange of continuous culture water, inclusion of local paddy field common carp and feed provision, contained GBCrC. In the breeding season, inclusion of local paddy field common carp was the major factor in selection of paddy field by GBCrC. No male individual was observed in all detected GBCrC, female individuals could mate with the local male paddy field common carps to produce fries, and all the fries represented the characteristics of the GBCrC. Genetic diversity analysis showed that the crucian carp had evolved into an independent population, while phylogenetic analysis demonstrated that GBCrC was clustered with several local crucian carp (LCrC) individuals, and other LCrC ones clustered with other geographical strains. Compared with other distribution areas of GBCrC, Pingzheng village (PZ) presented special temperatures, altitudes, sizes of GBCrC, fish reproduction and sales. The chromosome slide observation showed there were 150 chromosomes in GBCrC. Based on these findings, this study maintained that GBCrC, deriving from LCrC and originating from PZ, might adopt the reproductive mode of gynogenesis. The results of this study enrich the understanding of the traditional agricultural system.



中文翻译:

稻鱼一体化养殖鲫金背突变体的遗传和繁殖模式分析

传统农业体系展示了农民的耕作智慧,同时延伸了许多新的农业生态系统,孕育了许多新奇有趣的生物。本研究介绍了一种本土金背鲫鱼 (GBCrC; Carassius gibelio ) 的发现。)从全球重要的农业遗产系统之一侗族稻-鱼-鸭系统中,通过探索和分析其外部形态特征、分布、稻田选择、繁殖方式、遗传多样性和起源。调查发现,在6个村150个被调查的水田中,以连续养殖水交换、包括当地水田鲤鱼和饲料供应为特征的水田中约有27.33%含有GBCrC。在繁殖季节,纳入当地水田鲤鱼是GBCrC选择水田的主要因素。在所有检测到的GBCrC中均未观察到雄性个体,雌性个体可以与当地的雄性水田鲤鱼交配生产鱼苗,所有的鱼苗都代表了GBCrC的特征。遗传多样性分析表明,鲫鱼已经进化成一个独立的种群,而系统发育分析表明,GBCrC与几个当地的鲫鱼(LCrC)个体聚集在一起,而其他LCrC个体与其他地理品系聚集在一起。与其他GBCrC分布区相比,平征村(PZ)呈现出特殊的温度、海拔、GBCrC大小、鱼类繁殖和销售情况。染色体切片观察显示GBCrC中有150条染色体。基于这些发现,本研究认为源自 LCrC 和 PZ 的 GBCrC 可能采用雌核发育的生殖方式。本研究成果丰富了对传统农业系统的认识。而系统发育分析表明,GBCrC 与几个当地鲫鱼 (LCrC) 个体聚集在一起,而其他 LCrC 个体与其他地理品系聚集在一起。与其他GBCrC分布区相比,平征村(PZ)呈现出特殊的温度、海拔、GBCrC大小、鱼类繁殖和销售情况。染色体切片观察显示GBCrC中有150条染色体。基于这些发现,本研究认为源自 LCrC 和 PZ 的 GBCrC 可能采用雌核发育的生殖方式。本研究成果丰富了对传统农业系统的认识。而系统发育分析表明,GBCrC 与几个当地鲫鱼 (LCrC) 个体聚集在一起,而其他 LCrC 个体与其他地理品系聚集在一起。与其他GBCrC分布区相比,平征村(PZ)呈现出特殊的温度、海拔、GBCrC大小、鱼类繁殖和销售情况。染色体切片观察显示GBCrC中有150条染色体。基于这些发现,本研究认为源自 LCrC 和 PZ 的 GBCrC 可能采用雌核发育的生殖方式。本研究成果丰富了对传统农业系统的认识。平征村(PZ)展示了特殊的温度、海拔、GBCrC的大小、鱼类的繁殖和销售。染色体切片观察显示GBCrC中有150条染色体。基于这些发现,本研究认为源自 LCrC 和 PZ 的 GBCrC 可能采用雌核发育的生殖方式。本研究成果丰富了对传统农业系统的认识。平征村(PZ)展示了特殊的温度、海拔、GBCrC的大小、鱼类的繁殖和销售。染色体切片观察显示GBCrC中有150条染色体。基于这些发现,本研究认为源自 LCrC 和 PZ 的 GBCrC 可能采用雌核发育的生殖方式。本研究成果丰富了对传统农业系统的认识。

更新日期:2022-05-10
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